[Amylase production of Streptomyces rimosus TM-55 and their 2-deoxyglucose resistant mutants].

Abstract

Streptomyces rimosus TM-55 was treated with 3% ethyl melthylsulfonate for 60 minutes to generate mutants producing high amount of amylase, and 1,283 mutants were isolated from yeast extract starch (YS) medium containing 0.1% 2-deoxyglucose. Amylase activity was primarily screened by clear zone formation on YS medium after spraying with iodine solution. Two mutants designed as D-35 and D-62 had higher amylase activity than that of the parent strain. Amylase activity of the mutants in the YS broth was 2.97- and 3.45-fold of the parent strain, respectively. With the addition of 0.1% lactose, and 2-deoxyglucose, amylase activity of the mutants D-35 and D-62 was 27-73% and 4-7% higher than that of the parent strain. With the addition of 0.1% sucrose, amylase activity of the mutant D-35 was 18.37% lower than that of the parent strain. Moreover, with the supplement of 0.1% glucose, amylase activity of both the mutants D-35 and D-62 was 3.67 and 3.40 fold of the parent strain.