Dietary hydroperoxides of linoleic acid decompose to aldehydes in stomach before being absorbed into the body

Kazuki Kanazawa, Hitoshi Ashida
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引用次数: 111

Abstract

Our previous study (Biochim. Biophys. Acta 1393 (1998) 336–348, this issue) found that dietary hydroperoxides of trilinoleoylglycerol were broken down, releasing linoleic acid hydroperoxides (LA-OOH) in the stomach without reaching the intestines. The present paper describes the catabolic fate of LA-OOH in rat gastrointestines, in an attempt to elucidate those products which can be absorbed into the body. At an intragastric dose of 6.5 or 18 μmol, LA-OOH was not transported to the intestines as determined by HPLC. At large doses (200 or 800 μmol), much greater than that in the daily diet, there was partial leakage of LA-OOH to the intestines. The periodical fate was analyzed with 17.2 μmol [U-14C]LA-OOH chemically and radiochemically. Exemplifying the product composition at 30 min after treatment (as percentage of dosed amount), 27% unchanged LA-OOH, 9.7% epoxyketones, 3.5% hydroxyls (LA-OH), 2.4% decomposed aldehydes, and 13% unknown products were found in the gastric lumen. Another 25% was incorporated in the gastric tissue, and the other 6.4% occurred in the intestinal lumen and tissue as decomposed aldehyde. The LA-OH further decomposed to aldehydes with time in the stomach. When an aldehyde mixture was prepared and dosed, significant increases in hexanal and 4-hydroxynonenal were detected in the liver 15 h later. These results show that the dietary LA-OOH is decomposed to aldehydes in the stomach and that aldehydes are partly absorbed into the body.

膳食中亚油酸的氢过氧化物在被人体吸收之前会在胃中分解成醛类
我们之前的研究(biochem)。Biophys。Acta 1393(1998) 336-348,这期)发现饮食中的三油酰甘油氢过氧化物被分解,在胃中释放亚油酸氢过氧化物(LA-OOH),而不到达肠道。本文描述了LA-OOH在大鼠胃肠道中的分解代谢命运,试图阐明哪些产物可以被人体吸收。HPLC测定,在6.5或18 μmol的灌胃剂量下,LA-OOH没有被输送到肠道。在大剂量(200或800 μmol)下,远高于日常饮食,LA-OOH部分渗漏到肠道。用17.2 μmol [U-14C]LA-OOH进行化学和放射化学分析。以处理后30分钟的产物组成为例(以给药量的百分比),在胃腔中发现27%不变的LA-OOH, 9.7%的环氧酮,3.5%的羟基(LA-OH), 2.4%的分解醛和13%的未知产物。另有25%进入胃组织,6.4%以分解醛的形式进入肠腔和肠组织。随着时间的推移,LA-OH在胃中进一步分解成醛类。当制备醛混合物并给药时,15h后肝脏中己醛和4-羟基壬烯醛含量显著增加。上述结果表明,饲料中的LA-OOH在胃中分解为醛类,醛类被部分吸收到体内。
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