Clindamycin resistance transfer in Bacteroides fragilis.

L J Teng, S J Liaw, K T Luh, S W Ho
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Abstract

Clindamycin is one of the antimicrobial agents most commonly used against anaerobes. Resistance to clindamycin in Bacteroides fragilis has been increasing recently. Thirty strains of clindamycin-resistant (including multi-resistant) B. fragilis were collected for study of cross-resistance to beta-lactam agents and beta-lactam--beta-lactamase inhibitor and resistance transferability. Imipenem was the most active drug against these 30 isolates. Resistance to clindamycin was transferred to a recipient in 12 out of 30 donor strains by using filter-mating. Of 12 transconjugants, only three had detectable plasmids by alkaline lysis method and the remaining nine strains lacked plasmids. The transfer frequencies ranged from 10(-4) to 10(-6). The role of plasmid in the resistance transfer was not certain. However, the results suggest that non-plasmid-mediated transfer accounted for the majority of the transfers of clindamycin-resistance of B. fragilis in this study. Tetracycline resistance was co-transferred from six donors. There was no evidence of co-transference of beta-lactam resistance under the selection marker of clindamycin, beta-lactam, or both. Therefore, non-plasmid-mediated transfer may play an important role in dissemination of resistance transfer in B. fragilis in Taiwan.

脆弱拟杆菌的克林霉素耐药性转移。
克林霉素是抗厌氧菌最常用的抗菌剂之一。近年来脆弱拟杆菌对克林霉素的耐药性呈上升趋势。收集30株耐克林霉素(含多重耐药)脆弱芽孢杆菌,研究其对-内酰胺类药物和-内酰胺--内酰胺酶抑制剂的交叉耐药性及耐药性转移性。亚胺培南是对这30株分离株最有效的药物。30株供体菌株中有12株通过过滤配种将克林霉素耐药性转移到受体。12株转偶联菌中,碱法检测到质粒的仅有3株,其余9株质粒缺失。传递频率从10(-4)到10(-6)不等。质粒在抗性转移中的作用尚不确定。然而,结果表明,在本研究中,非质粒介导的转移占脆弱芽孢杆菌克林霉素耐药转移的大部分。四环素耐药性是由六名献血者共同转移的。在克林霉素、β -内酰胺或两者的选择标记下,没有证据表明β -内酰胺耐药共转移。因此,非质粒介导的转移可能在台湾脆弱芽孢杆菌的抗性转移传播中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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