Lipopolysaccharide binding and antibacterial activities of a synthetic peptide representing amino acids 90-101 of bactericidal/permeability-increasing protein.

T M Yeh, S C Chao, H C Chang
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Abstract

The bactericidal/permeability-increasing protein (BPI) of polymorphonuclear leukocytes is a potent antibacterial agent specific for gram-negative bacteria. BPI can bind to lipopolysaccharide (LPS) and neutralize its toxicity. However, little is known about the specific site and mechanisms of the BPI involved in this LPS binding and antibacterial activities. This study compared the amino acid sequences among BPI, cecropin A, magainin 2, and polymyxin B, and identified a common structure among these four bactericidal agents. They share a basic amphipathic alpha helix motif (Baah). A short peptide that represents amino acids 90-101 of BPI was then synthesized to test if it possessed any LPS binding and antibacterial activities. Results from in vitro lymphocyte culture indicated this peptide was able to inhibit LPS-induced lymphocyte proliferation, suggesting that it may interact with LPS. This LPS binding ability of BPI peptide 90-101 was further supported by the results from HPLC assays which showed the mobility of the peptide shifted in the presence of LPS. Furthermore, the antibacterial spectra of this peptide and cecropin peptide 1-11 were very similar to that of polymyxin B, even though the antibacterial activities of these two peptides were less potent than that of polymyxin B. In addition, the antibacterial activities of these two peptides and polymyxin B were inhibited by free LPS or a high concentration of MgCl2. These results thus suggest that a common structure (Baah) and antibacterial mechanism may be involved in these antibacterial agents.

代表抗菌/增透蛋白90-101氨基酸的合成肽的脂多糖结合和抗菌活性。
多形核白细胞的杀菌/通透性增加蛋白(BPI)是一种针对革兰氏阴性细菌的有效抗菌剂。BPI能与脂多糖(LPS)结合并中和其毒性。然而,BPI参与LPS结合和抗菌活性的具体位点和机制尚不清楚。本研究比较了BPI、cecropin A、magainin 2和多粘菌素B的氨基酸序列,确定了这四种杀菌剂的共同结构。它们共享一个基本的两性α螺旋motif (Baah)。然后合成一个代表BPI 90-101氨基酸的短肽,以测试其是否具有LPS结合和抗菌活性。体外淋巴细胞培养结果表明,该肽能够抑制LPS诱导的淋巴细胞增殖,提示其可能与LPS相互作用。高效液相色谱分析结果进一步证实了BPI肽90-101的LPS结合能力,结果显示肽的流动性在LPS存在下发生了变化。此外,该肽和天蚕肽1-11的抗菌光谱与多粘菌素B非常相似,尽管这两种肽的抗菌活性不如多粘菌素B。此外,这两种肽和多粘菌素B的抗菌活性受到游离LPS或高浓度MgCl2的抑制。这些结果表明,这些抗菌剂可能具有共同的结构(Baah)和抗菌机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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