HIV-1 reverse transcriptase is capable of elongating derivatives of sequence specific noncomplementary oligodeoxynucleotides.

I V Martyanov, O D Zakharova, E Sottofattori, G A Maksakova, D V Pyshnyi, M Mazzei, A Balbi, S Litvak, L Tarrago-Litvak, G A Nevinsky
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引用次数: 5

Abstract

We have carried out a comparison of KM and Vmax values for various primers in the polymerization reaction catalyzed by the HIV-1 RT. The affinity of RT for complementary d(pT)6 containing two different 5'-end pyranone derivatives was 2-3 orders of magnitude higher (KM = 3-15 nM) than that of d(pT)6 (KM = 12.6 mM). Oligodeoxynucleotides (ODNs) noncomplementary to poly(A) template were not elongated by RT. However, derivatives of d(CAGGTG) containing the 5'-terminal chromone and coumarin related groups were efficient primers showing KM (30-300 nM) and Vmax (75-93%) values comparable with that for d(pT)10 (800 nM; 100%). The [d(CAGGTG)]ddT ODN derivatives were effective inhibitors of RT. The primer function of derivatives of noncomplementary ODNs appears to be due to the additional interactions of their 5'-terminal groups with the enzyme tRNA-binding site.

HIV-1逆转录酶能够延长序列特异性非互补寡脱氧核苷酸的衍生物。
我们比较了HIV-1 RT催化聚合反应中不同引物的KM和Vmax值。RT对含有两种不同5'端吡喃酮衍生物的互补d(pT)6的亲和力(KM = 3-15 nM)比d(pT)6 (KM = 12.6 mM)高2-3个数量级(KM = 3-15 nM)。不与poly(A)模板互补的Oligodeoxynucleotides (ODNs)没有被rt延长。然而,含有5'端染色质和香豆素相关基团的d(CAGGTG)衍生物是有效的引物,其KM (30-300 nM)和Vmax(75-93%)值与d(pT)10 (800 nM)相当;100%)。[d(CAGGTG)]ddT ODN衍生物是rt的有效抑制剂。非互补ODN衍生物的引物功能似乎是由于它们的5'端基团与酶trna结合位点的额外相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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