Shu Wang , Yinghua Zhou , Agneta Lukinius , Kjell Öberg , Britt Skogseid , Anders Gobl
{"title":"Molecular cloning and characterization of a cDNA encoding mouse phospholipase C-β31","authors":"Shu Wang , Yinghua Zhou , Agneta Lukinius , Kjell Öberg , Britt Skogseid , Anders Gobl","doi":"10.1016/S0005-2760(98)00074-5","DOIUrl":null,"url":null,"abstract":"<div><p>A cDNA encoding mouse PLC-β3 (mPLC-β3) was identified by screening a mouse kidney cDNA library and using the rapid amplification of cDNA ends (RACE) method. The predicted open reading frame was 3705 bp in length. The deduced 1235 amino acid (aa) sequence shares 95.3% and 92% homology with the sequences of rat and human PLC-β3, respectively. The corresponding mRNA is highly expressed in kidney, skeletal muscle, liver, lung, heart and brain. In spleen, mPLC-β3 mRNA was not detectable, which is in contrast to humans where there is a distinct expression. Using ultrastructural immunocytochemistry, mPLC-β3 expression was detected in the heterochromatin of the nucleus in mouse brain neurons. The observation of PLC-β3 nuclear localization suggests that PLC-β3 may have intranuclear functions.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 1","pages":"Pages 173-178"},"PeriodicalIF":0.0000,"publicationDate":"1998-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00074-5","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0005276098000745","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
A cDNA encoding mouse PLC-β3 (mPLC-β3) was identified by screening a mouse kidney cDNA library and using the rapid amplification of cDNA ends (RACE) method. The predicted open reading frame was 3705 bp in length. The deduced 1235 amino acid (aa) sequence shares 95.3% and 92% homology with the sequences of rat and human PLC-β3, respectively. The corresponding mRNA is highly expressed in kidney, skeletal muscle, liver, lung, heart and brain. In spleen, mPLC-β3 mRNA was not detectable, which is in contrast to humans where there is a distinct expression. Using ultrastructural immunocytochemistry, mPLC-β3 expression was detected in the heterochromatin of the nucleus in mouse brain neurons. The observation of PLC-β3 nuclear localization suggests that PLC-β3 may have intranuclear functions.
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