Intrapopulation variation in ecological energetics of the garter snake Thamnophis sirtalis, with analysis of the precision of doubly labeled water measurements.

Abstract

The evolution of energetics must begin with variation within populations in ecologically realized rates of energy acquisition and expenditure. We measured aspects of field energy budgets (including metabolic rates, feeding rates, and growth rates) in a large sample of free-living garter snakes (Thamnophis sirtalis) from a single temperate/mesic population in northwestern California during their summer active season. We then analyzed interindividual variation for correlations among variables and patterns attributable to body size and sex. Field metabolic rates (measured with use of doubly labeled water) scaled in direct proportion to body mass. These rates of field energy expenditure were higher (both in absolute terms and in relation to resting metabolic rates) than those previously measured in snakes and iguanian lizards and were similar to those reported for highly active, widely foraging scincomorphan lizards. Feeding rates (as indexed by water influx rates) and growth rates were correspondingly high compared to those of other squamate reptiles. We found considerable residual variation in all measured variables not attributable to body size. Effects of sex were detected for water influx and growth rates (females > males), but not for field metabolic rate. Individual field metabolic rate was apparently consistent (repeatable) over time, water influx rate was not, and individual growth rates were strongly negatively correlated over two sequential time periods. We were unable to detect convincing correlations between any individual measures of field energetics and any commonly measured, standard laboratory measurements of oxygen consumption (standard metabolic rate at two body temperatures and maximal oxygen consumption for exercise) made on the same individuals. However, body-size-independent field rates of energy expenditure, energy intake, and growth were strongly and positively intercorrelated among individuals. We attribute these patterns to an overriding effect of costs associated with digestion and growth on field energetics, such that individual snakes that were effective foragers achieved high feeding rates and, hence, high growth rates, but also incurred high costs of growth and digestion that largely determined field metabolic rate.