The effect of glutamate and inhibitors of NMDA receptors on postdenervation decrease of membrane potential in rat diaphragm.

A Kh Urazaev, N V Naumenko, G I Poletayev, E E Nikolsky, F Vyskocil
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引用次数: 13

Abstract

The early postdenervation depolarization of rat diaphragm muscle fibers (8-10 mV within 3 h in vitro) is substantially smaller (3 mV) when muscles are bathed with 1 x 10(-3) M L-glutamate (Glu) or 1 x 10(-3) M N-methyl-D-aspartate (NMDA). The effects of Glu and NMDA are inhibited in a dose-dependent manner by competitive inhibitor 2-amino-5-phosphonovaleric acid (APV) with Ki 6.3 x 10(-4) M, by 2 x 10(-7) M MK-801, which acts as an open channel inhibitor, by 2-3 x 10(-4) Zn2+, which reacts with surface-located sites of the NMDA subtype of the glutamate receptor, and also by glycine-free solutions and 7-Cl-kynurenic acid, which inhibits the glycine binding sites on NMDA receptors. It follows that the effect of glutamate on early post-denervation depolarization is mediated by the NMDA subtype of glutamate receptor with similar pharmacological properties to those found in neurons. The only exception found was the glutamate-like action of 1 x 10(-7) M MK-801, which partially prevented the early postdenervation depolarization when present in the muscle bath during the first 3 h after nerve section.

谷氨酸和NMDA受体抑制剂对大鼠膈肌去神经后膜电位下降的影响。
用1 × 10(-3) M l-谷氨酸(Glu)或1 × 10(-3) M n -甲基- d -天冬氨酸(NMDA)浸泡肌肉时,大鼠膈肌纤维的早期去神经支配后去极化(体外3小时内8-10 mV)明显较小(3 mV)。Glu和NMDA的作用以剂量依赖性的方式被具有Ki 6.3 × 10(-4) M的竞争性抑制剂2-氨基-5-磷酸戊酸(APV)、作为开放通道抑制剂的2 × 10(-7) M MK-801、与谷氨酸受体的NMDA亚型表面位点反应的2-3 × 10(-4) Zn2+以及无甘氨酸溶液和抑制NMDA受体上甘氨酸结合位点的7-Cl-kynurenic酸所抑制。由此可见,谷氨酸对早期去神经支配后去极化的影响是由谷氨酸受体的NMDA亚型介导的,其药理特性与神经元中发现的相似。唯一的例外是1 × 10(-7) M MK-801的谷氨酸样作用,在神经切片后的前3小时内出现在肌肉浴中,部分阻止了早期去神经后去极化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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