Electrospray ionization tandem mass spectrometric analysis of sulfatide.

Fong-Fu Hsu, Alan Bohrer, John Turk
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引用次数: 113

Abstract

The sphingolipid sulfatide is a component of myelin and some non-neuronal cells. Antibodies to sulfatide occur in some patients with autoimmune neuropathies and in patients with insulin-dependent diabetes mellitus (IDDM) caused by immunologic destruction of insulin-secreting pancreatic islet β-cells. Distinct sulfatide molecular species may differ in immunogenicity, and facile means to identify sulfatide species in islets and other tissues obtainable in only small amounts could be useful. Electrospray ionization mass spectrometry (ESI/MS) permits structural determination of small quantities of phospholipids and is applied here to sulfatide analysis. We find that sulfatide standards are readily analyzed by negative ion ESI/MS, and tandem mass spectra of individual species exhibit some ions common to all species and other ions that reflect distinct fatty acid substituents in different sulfatide molecules. A signature ion cluster resulting from cleavage directed by the α-hydroxy group of sulfatide species with a hydroxylated fatty acid substituent identifies such species. Sulfatide profiles in tissue lipid extracts can be obtained by ESI/MS/MS scanning for common sulfatide ions and for ions reflecting fatty acid substituents. Islets are demonstrated to contain sulfatide and to exhibit a profile of species different from that of brain.

硫脂的电喷雾电离串联质谱分析。
鞘脂硫脂是髓磷脂和一些非神经元细胞的组成成分。一些自身免疫性神经病变患者和胰岛素依赖型糖尿病(IDDM)患者中可出现硫脂抗体,这是由分泌胰岛素的胰岛β细胞的免疫破坏引起的。不同的硫脂分子种类可能在免疫原性上有所不同,而在胰岛和其他组织中识别少量硫脂的简便方法可能是有用的。电喷雾电离质谱法(ESI/MS)允许少量磷脂的结构测定,并应用于硫脂分析。我们发现,用负离子ESI/MS可以很容易地分析硫脂标准物,单个物种的串联质谱显示出一些所有物种共有的离子和其他离子,这些离子反映了不同硫脂分子中不同的脂肪酸取代基。由硫脂类α-羟基与羟基化脂肪酸取代基直接裂解产生的特征离子簇识别了这些物种。通过ESI/MS/MS扫描,可以获得组织脂质提取物中常见的硫脂质离子和反映脂肪酸取代基的离子。胰岛被证明含有硫脂,并表现出与大脑不同的物种特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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