Preferential expression of cholesteryl ester transfer protein mRNA by stromal-vascular cells of human adipose tissue

Thierry Radeau, Malcolm Robb, Michael McDonnell, Ruth McPherson
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引用次数: 16

Abstract

Cholesteryl ester transfer protein (CETP) mRNA is more abundantly expressed in small mature adipocytes as compared to large, lipid-rich adipocytes [Radeau et al., J. Lipid Res. 36 (1995) 2552–2561]. In the present study, the stromal vascular fraction (SVF) of human adipose tissue was isolated and the presence of very small fat cells in this fraction confirmed by electron microscopy and by demonstrating the presence of mRNA for adipsin and for CCAAT enhancer binding protein α (C/EBPα), a marker of adipocyte differentiation. sn-Glycerol 3-phosphate dehydrogenase (GPDH) activity was present in the SVF but not in the preadipocyte fraction. Northern blot analysis of human adipose tissue demonstrated that CETP mRNA expression was significantly greater (+96%, P<0.03) in stromal-vascular cells (SVC) as compared to mature fat cells. By comparison, lipoprotein lipase mRNA expression was lower (−75%, P<0.03) in SVC while apolipoprotein E mRNA expression was not significantly different in SVC as compared to isolated adipocytes. By RT-PCR analysis, we demonstrated that CETP mRNA was expressed by human pre-adipocytes at levels less than those of SVC and adipocytes. The absence of monocytes/macrophages in SVC was confirmed by the absence of FcγRIII (CD16) mRNA in these fractions. These data demonstrate that CETP mRNA is most highly expressed in the immature fat cells of human adipose tissue, consistent with other experiments from this laboratory demonstrating that CETP plays an important local role in adipocyte cholesterol accumulation.

人脂肪组织基质血管细胞对胆固醇酯转移蛋白mRNA的优先表达
胆固醇酯转移蛋白(CETP) mRNA在小的成熟脂肪细胞中比大的富含脂质的脂肪细胞表达更丰富[Radeau等,J.脂质研究,36(1995):2552-2561]。在本研究中,分离了人脂肪组织的基质血管部分(SVF),并通过电镜和脂肪素mRNA和脂肪细胞分化标志物CCAAT增强子结合蛋白α (C/EBPα) mRNA的存在证实了该部分中存在非常小的脂肪细胞。sn-甘油3-磷酸脱氢酶(GPDH)活性存在于SVF中,而不存在于脂肪前细胞部分。人体脂肪组织的Northern blot分析表明,与成熟脂肪细胞相比,基质血管细胞(SVC)中CETP mRNA的表达显著增加(+96%,P<0.03)。相比之下,脂蛋白脂肪酶mRNA在SVC中的表达较低(- 75%,P<0.03),而载脂蛋白E mRNA在SVC中的表达与分离的脂肪细胞相比无显著差异。通过RT-PCR分析,我们发现CETP mRNA在人类脂肪前细胞中的表达水平低于SVC和脂肪细胞。在SVC中,单核/巨噬细胞的缺失被证实为FcγRIII (CD16) mRNA的缺失。这些数据表明,CETP mRNA在人类脂肪组织的未成熟脂肪细胞中表达最高,与本实验室的其他实验一致,表明CETP在脂肪细胞胆固醇积累中起着重要的局部作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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