[CD4+ alpha beta T cell and gamma delta T cell responses to BCG in patients with pulmonary tuberculosis--comparison with healthy controls].

Nihon Kyobu Shikkan Gakkai zasshi Pub Date : 1997-12-01
K Tsukaguchi, H Okamura, T Tokuyama, Y Okamoto, A Fu, C Yamamoto, M Nakaya, A Kobayashi, T Yoneda, N Narita
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Abstract

We demonstrated that CD4+ alpha beta (CD4+) and gamma delta T cell subsets from healthy donors had similar effector functions (cytotoxicity and cytokine production) in response to mycobacterial antigens, despite differences in the antigens recognized. To elucidate the pathogenesis of pulmonary tuberculosis, this study was undertaken to compare T cell functions between patients with pulmonary tuberculosis with no complications and healthy controls. Both resting and activated CD4+ and gamma delta T cells from the patient group proliferated in response to live BCG at a significantly lower rate than those from the control group. The cytotoxicity of BCG-pulsed monocytes and IFN-gamma production in both the CD4+ and gamma delta T cells from patients was significantly lower than those of controls. In contrast to IFN-gamma, significantly higher IL-10 production by both CD4+ and gamma delta T cells from patients was detected. The proliferative responses to BCG by CD4+ and gamma delta T cells from patients after antituberculous therapy were partially restored, but remained at lower levels compared with controls. These results suggest that not only a general deterioration in CD4+ and gamma delta T cells effector functions, but also suppressive factors (such as IL-10) might be responsible for the pathogenesis of pulmonary tuberculosis, and that the low response to BCG by both CD4+ and gamma delta T cells in patients with tuberculosis is in part attributable to patient predisposition.

[肺结核患者CD4+ α β T细胞和γ δ T细胞对卡介苗的反应——与健康对照比较]。
我们证明了来自健康供体的CD4+ α - β (CD4+)和γ δ T细胞亚群在对分枝杆菌抗原的反应中具有相似的效应功能(细胞毒性和细胞因子产生),尽管识别的抗原存在差异。为了阐明肺结核的发病机制,本研究对无并发症肺结核患者和健康对照者的T细胞功能进行了比较。患者组的静止和激活的CD4+和γ δ T细胞对活BCG的增殖反应明显低于对照组。bcg脉冲单核细胞的细胞毒性以及患者CD4+和γ δ T细胞中ifn - γ的产生均显著低于对照组。与ifn - γ相反,检测到患者CD4+和γ δ T细胞产生的IL-10显著增加。抗结核治疗后患者的CD4+和γ δ T细胞对卡介苗的增殖反应部分恢复,但与对照组相比仍处于较低水平。这些结果表明,不仅CD4+和γ δ T细胞效应功能的普遍恶化,而且抑制因子(如IL-10)可能与肺结核的发病机制有关,并且结核病患者CD4+和γ δ T细胞对BCG的低反应部分可归因于患者易感性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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