Different effects of anesthetics on spontaneous leukocyte rolling in rat skin.

G H Janssen, G J Tangelder, M G oude Egbrink, R S Reneman
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引用次数: 24

Abstract

In immunological reactions, leukocytes need to travel from the intravascular space through the vessel wall into the surrounding tissue. The first step in this process is leukocyte rolling, which has often been studied in anesthetized animals. In this study, we investigated the effect of pentobarbital, Hypnorm and both components of the latter, fentanyl and fluanisone, on this primary leukocyte-endothelial cell interaction. Using intravital brightfield video microscopy, observations were made in postcapillary venules in the intact skin of the nailfold of trained conscious Lewis rats. Subsequently, the animals were anesthetized and observations were made in vivo. Leukocyte rolling was significantly elevated after injection of Hypnorm or fentanyl, while pentobarbital and fluanisone had no effect. None of the anesthetics affected leukocyte rolling velocity. Blood flow was significantly increased only after injection of Hypnorm and fluanisone. No correlation existed between the relative changes in leukocyte rolling and concomitant changes in blood flow. The results show that the level of leukocyte rolling can be affected by anesthetics. These changes are probably not mediated by changes in local hemodynamics. Pentobarbital anesthesia does not influence leukocyte rolling. Therefore, pentobarbital is a suitable anesthetic for observation of leukocyte rolling in skin. Hypnorm significantly increases the level of rolling in skin venules. This effect seems to be caused mainly by fentanyl.

麻醉药对大鼠皮肤自发白细胞滚动的不同影响。
在免疫反应中,白细胞需要从血管内空间穿过血管壁进入周围组织。这个过程的第一步是白细胞滚动,这经常在麻醉动物中进行研究。在这项研究中,我们研究了戊巴比妥、Hypnorm以及后者的两种成分芬太尼和氟尼松对这种原发性白细胞-内皮细胞相互作用的影响。采用活体明视野视频显微镜对Lewis大鼠完整甲襞皮肤毛细血管后小静脉进行了观察。随后,对动物进行麻醉,在体内进行观察。注射Hypnorm或芬太尼后白细胞滚动明显升高,而戊巴比妥和氟尼松对白细胞滚动无影响。所有麻醉药均不影响白细胞滚动速度。仅注射Hypnorm和fluanisone后血流量明显增加。白细胞滚动的相对变化与伴随的血流变化之间不存在相关性。结果表明,麻醉剂可影响白细胞滚动水平。这些变化可能不是由局部血流动力学的改变介导的。戊巴比妥麻醉不影响白细胞滚动。因此,戊巴比妥是观察皮肤白细胞滚动的合适麻醉剂。Hypnorm显著增加皮肤小静脉的滚动水平。这种效果似乎主要是由芬太尼引起的。
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