Changes in sequestered leukocytes and platelets in the pulmonary microvasculature of rats with monocrotaline-induced pulmonary hypertension.

International journal of microcirculation, clinical and experimental Pub Date : 1997-11-01 DOI:10.1159/000179243

S Kato, N Ohnuma, K Ohno, K Takasaki, S Okamoto, T Asai, M Okuda, T Nakamoto, M Iizuka

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引用次数: 5

Abstract

The role of leukocytes (WBCs) and platelets (PLTs) in the pulmonary circulation may be important in the development of monocrotaline (MCT)-induced pulmonary hypertension in rats. We investigated the changes in WBCs and PLTs in the pulmonary microvasculature during the development of chronic pulmonary hypertension in MCT rats by real-time confocal scanning laser microscopy. The number of WBCs sequestered in the pulmonary microvasculature increased significantly from day 7 after MCT injection, but no further increase occurred from days 14-28. The number of PLTs sequestered in the pulmonary microvasculature increased significantly from day 7 after MCT injection, and reached a peak on day 14. However, the number of PLTs sequestered on days 21 and 28 after MCT injection was significantly lower than on day 14. These findings suggest that PLTs mainly contribute to the initial and middle stages of the development of MCT-induced pulmonary hypertension in rats, while WBCs mainly contribute to the middle and late stages.

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单芥碱诱导肺动脉高压大鼠肺微血管中隔离白细胞和血小板的变化。

白细胞(wbc)和血小板(PLTs)在肺循环中的作用可能在大鼠单氯胆碱(MCT)诱导的肺动脉高压的发展中很重要。采用实时共聚焦扫描激光显微镜观察MCT大鼠慢性肺动脉高压发生过程中肺微血管白细胞和血小板的变化。从注射MCT后第7天开始，肺微血管中隔离的白细胞数量显著增加，但从第14-28天开始没有进一步增加。从注射MCT后第7天开始，肺微血管中隔离的plt数量显著增加，并在第14天达到峰值。然而，注射MCT后第21天和第28天的plt数量明显低于第14天。这些结果表明，plt主要参与mct诱导的大鼠肺动脉高压发展的初期和中期，而wbc主要参与中晚期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of microcirculation, clinical and experimental

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