Q-RT-PCR: data analysis software for measurement of gene expression by competitive RT-PCR.

Computer applications in the biosciences : CABIOS Pub Date : 1997-12-01 DOI:10.1093/bioinformatics/13.6.587

P A Doris, A Hayward-Lester, J K Hays

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Abstract

Motivation: We have developed software to assist in the computation of gene expression from data obtained in competitive reverse transcription-polymerase chain reaction (RT-PCR). This report describes the mathematical basis of competitive RT-PCR and discusses the criteria which must be met to permit accurate estimations of gene expression to be obtained using this technique.

Results: The software that has been developed assists in both the assessment of assay performance (specifically in establishing the equality of amplification efficiency of the native and competitor templates) and in the routine analysis of data obtained in quantitation of gene expression by competitive RT-PCR. The software is a 100 kb module which functions as a Microsoft Excel add-in. It is compatible with both Windows and Mac versions of Excel 5 and Excel 7 on the Windows 95 platform, and employs the spreadsheet, statistical and graphing capabilities incorporated into Excel.

Availability: The software can be downloaded from http://www.grad.ttuhsc.edu/archive/. A brief summary in both HTML and Microsoft Word 6 format of the installation and use of the software is also located at this website.

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Q-RT-PCR:通过竞争性RT-PCR测量基因表达的数据分析软件。

动机:我们开发了软件来帮助计算从竞争性逆转录聚合酶链反应(RT-PCR)中获得的数据的基因表达。本报告描述了竞争性RT-PCR的数学基础，并讨论了必须满足的标准，以便使用该技术获得准确的基因表达估计。结果:已开发的软件有助于分析性能的评估(特别是在建立本地和竞争对手模板的扩增效率的平等)，并有助于通过竞争RT-PCR定量基因表达获得的数据的常规分析。该软件是一个100 kb的模块，功能为微软Excel插件。它在Windows 95平台上兼容Windows和Mac版本的Excel 5和Excel 7，并采用Excel中包含的电子表格、统计和绘图功能。可用性:软件可从http://www.grad.ttuhsc.edu/archive/下载。本网页亦以HTML及microsoftword6格式简述软件的安装及使用方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Computer applications in the biosciences : CABIOS

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