Antibodies against a furosemide binding protein from Ehrlich ascites tumour cells inhibit Na+, K+, Cl- co-transport in frog retinal pigment epithelium.

Acta ophthalmologica Scandinavica Pub Date : 1997-08-01 DOI:10.1111/j.1600-0420.1997.tb00399.x

M la Cour, A Baekgaard, T Zeuthen

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引用次数: 5

Abstract

Purpose: To investigate whether antibodies against a 100 kDa protein purified by furosemide affinity chromatography from Ehrlich ascites tumour cells could inhibit Na+, K+, Cl- co-transport in the isolated frog retinal pigment epithelium.

Methods: The rate of Na+, K+, Cl- co-transport across the retinal membrane in the isolated frog RPE preparation was measured as the rate of decrease in the intracellular Cl- activity observed after administration of furosemide in the apical bath. The intracellular Cl- activity was measured with double barrelled Cl- sensitive microelectrodes.

Results: Incubation of frog retinal pigment epithelium for 30 min with antibodies reduced the rate of Na+, K+, Cl- co-transport by 43%, while leaving all other measured electrophysiological parameters intact.

Conclusion: The antibodies inhibit Na+,K+,Cl- co-transport in the frog retinal pigment epithelium. This could be due to binding of the antibodies to the co-transporter itself or to a regulatory protein.

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来自埃利希腹水肿瘤细胞的抗速尿结合蛋白抗体可抑制蛙视网膜色素上皮中Na+、K+、Cl-的共转运。

目的:研究从埃利希腹水肿瘤细胞中纯化的抗100 kDa蛋白的抗体是否能抑制离体青蛙视网膜色素上皮中Na+、K+、Cl-的共转运。方法:用尖泡中给予速尿后观察细胞内Cl-活性的降低率来测定离体蛙RPE制剂中Na+、K+、Cl-在视网膜膜上的共转运率。用双管Cl敏感微电极测定细胞内Cl活性。结果:蛙视网膜色素上皮与抗体孵育30分钟，使Na+、K+、Cl-共转运率降低43%，而其他电生理参数保持不变。结论:该抗体可抑制蛙视网膜色素上皮内Na+、K+、Cl-的共转运。这可能是由于抗体与共转运体本身或调节蛋白结合所致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta ophthalmologica Scandinavica

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