The effect of pituitary adenylate cyclase activating polypeptide (PACAP) on amylase secretion from guinea pig pancreatic acini.

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1995-01-01

M Kitagawa, S Naruse, H Ishiguro, T Hayakawa, K Nokihara

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Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a novel hypothalamic peptide, structurally related to vasoactive intestinal peptide (VIP). Our previous study in conscious dogs revealed that PACAP stimulated exocrine pancreatic secretion in a manner different from VIP. The objectives of this study were to characterize the effects of PACAP on amylase secretion and intracellular cAMP production in guinea pig pancreatic acini and to compare them with those of VIP and secretin. PACAP38 and PACAP27 (10(-10)-10(-8)M) stimulated amylase secretion from pancreatic acini in a concentration-related manner. The order of potency for amylase secretion was PACAP27 = VIP > PACAP38. The maximally stimulated amylase secretion by PACAP27 was not enhanced by VIP or secretin, but was synergistically increased by cholecystokinin and A23187. PACAP38 and PACAP27 (10(-2)-10(-7)M) increased intracellular cAMP levels in a concentration-related manner. The potency for cAMP production was PACAP38 = PACAP27 = VIP. These results suggest that PACAP38 and PACAP27, like VIP, directly stimulate amylase secretion from guinea pig pancreatic acini through alterations in cellular cAMP levels.

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垂体腺苷酸环化酶激活多肽(PACAP)对豚鼠胰腺腺泡淀粉酶分泌的影响。

垂体腺苷酸环化酶激活多肽(PACAP)是一种与血管活性肠肽(VIP)结构相关的新型下丘脑肽。我们之前对有意识的狗的研究表明，PACAP刺激胰腺外分泌的方式与VIP不同。本研究的目的是表征PACAP对豚鼠胰腺腺泡淀粉酶分泌和细胞内cAMP生成的影响，并将其与VIP和分泌素的影响进行比较。PACAP38和PACAP27 (10(-10)-10(-8)M)以浓度相关的方式刺激胰腺腺泡淀粉酶分泌。淀粉酶分泌效价顺序为PACAP27 = VIP > PACAP38。PACAP27最大促淀粉酶分泌不受VIP和分泌素的增强，而受胆囊收缩素和A23187的协同增强。PACAP38和PACAP27 (10(-2)-10(-7)M)以浓度相关的方式增加细胞内cAMP水平。cAMP的效价为PACAP38 = PACAP27 = VIP。这些结果表明，PACAP38和PACAP27与VIP一样，通过改变细胞cAMP水平直接刺激豚鼠胰腺腺泡淀粉酶分泌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity

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