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Rat Kidney Thromboxane Synthase: cDNA Cloning and Gene Expression Regulation in Hydronephrotic Kidney*

Prostaglandins Pub Date : 1997-06-01 DOI:10.1016/S0090-6980(97)00059-2
Eikatsu Tsutsumi, Kazuhisa Takeuchi, Takaaki Abe, Nobuyuki Takahashi, Taro Kato, Yoshihiro Taniyama, Yukio Ikeda, Sadayoshi Ito, Keishi Abe
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引用次数: 9

Abstract

We isolated a rat homolog of thromboxane (TX) synthase cDNA (∼ 1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up-regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.

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大鼠肾血栓素合成酶:cDNA克隆及在肾积水中的表达调控*
我们以胎盘cDNA为模板，用聚合酶链反应扩增出的人TX合成酶cDNA片段，从肾脏中分离出大鼠血栓素(TX)合成酶cDNA同源物(约1.8 kb)。Northern blot分析显示大鼠TX合成酶基因在肺、肝、子宫中大量表达;中度见于肾脏。TX合成酶mRNA在输尿管结扎所致肾积水中表达上调。总之，我们揭示了大鼠肾TX合酶的结构。肾TX合成酶的上调可刺激TX合成，可能与水肾组织损伤有关。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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Prostaglandins
Prostaglandins
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