Indomethacin Depresses Prostaglandin F2α-Induced Contraction in Guinea-Pig Uterine Artery with Both Intact and Denuded Endoth

Prostaglandins Pub Date : 1997-06-01 DOI:10.1016/S0090-6980(97)00055-5

Leposava Grbović, Aleksandar Jovanović

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引用次数: 8

Abstract

The purpose of this study was to explore whether cyclooxygenase products derived from endothelium or vascular muscle participate in the response of guinea-pig uterine arterial rings to prostaglandin F_2α (PGF_2α). Contraction to PGF_2α (0.1–30 μM) occurred with and without endothelium at similar potency and efficacy (pEC₅₀ (−log EC₅₀) values respectively 5.87 ± 0.06 and 5.97 ± 0.07; maximal response respectively 78.1 ± 1.3% and 76.9 ± 1.5% of contraction induced by 126 mM KCl). Indomethacin (3–30 μM) suppressed the maximum response to PGF_2α and induced a rightward shift of concentration-response curves, regardless of the presence of endothelium. pIC₅₀ values for indomethacin were 4.67 and 4.74 for vessels with and without endothelium, respectively. In contrast, the thromboxane synthesis inhibitor OKY-046 (10 and 100 μM) did not affect the response to PGF_2α. We conclude that the PGF_2α-induced contraction in guinea-pig uterine artery is mediated, at least in part, through constrictor non-thromboxane prostanoid(s) of vascular muscle origin.

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吲哚美辛抑制前列腺素f2 α-诱导的豚鼠内膜完整和脱落子宫动脉收缩

本研究旨在探讨内皮细胞或血管肌生成的环加氧酶产物是否参与了豚鼠子宫动脉环对前列腺素F2α (PGF2α)的反应。有内皮和无内皮对PGF2α (0.1 ~ 30 μM)的收缩作用相似(pEC50 (- log EC50)值分别为5.87±0.06和5.97±0.07;最大反应分别为126 mM KCl诱导收缩的78.1±1.3%和76.9±1.5%)。吲哚美辛(3-30 μM)抑制了PGF2α的最大反应，诱导浓度-反应曲线向右移动，而不考虑内皮的存在。吲哚美辛在有内皮和无内皮血管中的pIC50值分别为4.67和4.74。相比之下，血栓素合成抑制剂OKY-046 (10 μM和100 μM)对PGF2α的反应没有影响。我们得出结论，pgf2 α-诱导的豚鼠子宫动脉收缩至少部分是通过血管肌来源的收缩剂非血栓素前列腺素介导的。

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Prostaglandins

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