Influence of dietary selenium on the disposition of arsenate in the female B6C3F1 mouse.

E M Kenyon, M F Hughes, O A Levander
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引用次数: 63

Abstract

Interactions between arsenic (As) and selenium (Se) at the metabolic level are multifaceted and complex. These interactions are of practical significance because populations in various parts of the world are simultaneously exposed to inorganic As in drinking water and Se mainly in the diet at varying levels. The primary goal of this study was to investigate whether differing dietary Se status would alter the profile of urinary metabolites or their time course for elimination after exposure to arsenate [As(V)]. Weanling female B6C3F1 mice were maintained for 28 d on either a control diet of powdered rodent meal sufficient in Se (A, 0.2 ppm) or Torula yeast-based (TYB) diets deficient (B, 0.02 ppm Se), sufficient (C, 0.2 ppm Se), or excessive (D, 2.0 ppm Se) in Se; mice then received by oral gavage 5 mg (As)/kg as sodium [73As] arsenate. The time course for elimination of total arsenic and metabolites in urine was measured over a 48-h period, and total arsenic was determined in feces and tissues at 48 h. Mice on the Se excess diet excreted a significantly higher percentage of urinary As as inorganic As, with a significantly decreased ratio of organic to inorganic As compared to Se-sufficient mice, suggesting that As methylation was decreased. Mice on the Se-deficient diet appeared to eliminate As(V), arsenite, and dimethylarsinic acid (DMA) in urine more slowly than Se-sufficient mice; however, further studies are required to confirm this finding. Mice on the Se-sufficient meal diet (A) excreted significantly less (by percent) arsenate-derived radioactivity in urine and more in feces compared to mice on the Se-sufficient TYB diet (C), with total elimination being similar for both groups. This indicates that mice on the meal diet absorbed significantly less As(V) than mice on the TYB diet, and this may be due to more fiber or "bulk" in the meal diet. This finding emphasizes the importance of considering dietary composition when interpreting and comparing As disposition studies. Overall this study provides suggestive evidence that dietary Se status alters As metabolism and disposition. This indicates that dietary Se status may be an issue that should be considered in the design and interpretation of epidemiologic studies.

膳食硒对雌性B6C3F1小鼠体内砷酸盐代谢的影响。
砷(As)和硒(Se)在代谢水平上的相互作用是多方面和复杂的。这些相互作用具有实际意义,因为世界各地的人口同时暴露于饮用水中的无机砷和主要来自饮食中的不同水平的硒。本研究的主要目的是调查不同饮食硒水平是否会改变暴露于砷酸盐后尿液代谢物的特征或其消除的时间过程[As(V)]。断奶雌性B6C3F1小鼠分别饲喂硒含量充足(a, 0.2 ppm)的鼠粉状饲料或硒含量不足(B, 0.02 ppm)、充足(C, 0.2 ppm)或过量(d, 2.0 ppm)的托尔拉酵母(TYB)饲料28 d;小鼠灌胃5 mg (As)/kg砷酸钠[73As]。在48小时内测量尿中总砷和代谢物的消除时间,并在48小时内测定粪便和组织中的总砷。与硒充足的小鼠相比,硒过量小鼠排出的尿液中无机砷的比例显著高于有机砷,有机砷与无机砷的比例显著降低,表明砷甲基化程度降低。缺硒小鼠的尿中砷(V)、亚砷酸盐和二甲基硅酸(DMA)的清除速度比富硒小鼠慢;然而,需要进一步的研究来证实这一发现。与摄入足硒的TYB饮食(C)的小鼠相比,摄入足硒膳食(A)的小鼠在尿液中排出的砷酸盐衍生放射性物质显著减少(百分比),而在粪便中排出的放射性物质则更多,两组的总消除量相似。这表明,膳食中的小鼠吸收的As(V)明显少于TYB饮食中的小鼠,这可能是由于膳食中含有更多的纤维或“散装”。这一发现强调了在解释和比较As倾向研究时考虑饮食组成的重要性。总的来说,本研究为膳食硒水平改变砷的代谢和处置提供了启发性证据。这表明在流行病学研究的设计和解释中,饮食硒水平可能是一个应该考虑的问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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