Differential induction of polyamine oxidase activity in liver and heart of iron-overloaded rats.

U R Tipnis, G Y He, M F Khan
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引用次数: 10

Abstract

The present study was undertaken to investigate the effect of iron dextran treatment on polyamine oxidase (PAO) activity, iron accumulation, and lipid peroxidation in livers and hearts of rats. PAO catalyzes oxidative deamination of polyamines, the cellular aliphatic cations. This reaction produces highly toxic hydrogen peroxide, 3-acetamidopropanal, and precursors of higher polyamines. The rats were given iron dextran daily for 7 d. In iron-dextran-treated rats, a marked increase in the hepatic level of iron was associated with enhanced lipid peroxidation and increased PAO activity. Though iron accumulation and lipid peroxidation in the iron-treated rats increased significantly in the heart, PAO activity remained unchanged. The paraffin sections of livers stained with Perls iron stain showed the presence of iron in macrophages and hepatocytes. The sections of hearts showed iron deposits only in macrophages, while myocytes showed no iron staining. These results show that although iron dextran treatment results in accumulation of iron in both liver and heart, it induces PAO activity only in liver. The significance of increased PAO activity in lipid peroxidation and fibrosis in iron-mediated injury is discussed.

铁超载大鼠肝脏和心脏中多胺氧化酶活性的不同诱导作用
本研究旨在探讨右旋糖酐铁处理对大鼠肝脏和心脏中多胺氧化酶(PAO)活性、铁积累和脂质过氧化的影响。PAO 催化细胞脂肪族阳离子多胺的氧化脱氨反应。这种反应会产生毒性很强的过氧化氢、3-乙酰胺基丙醛和高级多胺的前体。每天给大鼠服用右旋糖酐铁,连续 7 天。在服用右旋糖酐铁的大鼠中,肝脏中铁含量的显著增加与脂质过氧化反应的增强和 PAO 活性的增加有关。虽然铁处理大鼠心脏中的铁积累和脂质过氧化显著增加,但 PAO 活性保持不变。用 Perls 铁染色剂染色的肝脏石蜡切片显示,巨噬细胞和肝细胞中存在铁。心脏切片显示只有巨噬细胞中有铁沉积,而心肌细胞没有铁染色。这些结果表明,虽然右旋糖酐铁处理会导致肝脏和心脏中铁的积累,但它只在肝脏中诱导 PAO 活性。本文讨论了铁介导的损伤中 PAO 活性增加对脂质过氧化和纤维化的重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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