Effect of Leukotriene D4 on Ciliary Activity in Human, Guinea-Pig and Rat Respiratory Mucosa

Pulmonary pharmacology Pub Date : 1996-08-01 DOI:10.1006/pulp.1996.0029

Susanna Joki , Veijo Saano , Tommi Koskela , Elina Toskala , Michael A. Bray , Juhani Nuutinen

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引用次数: 15

Abstract

Leukotriene D4 (LTD4) is a potent bronchoconstrictor and inflammatory mediator in asthma. Data concerning the effects of LTD4 on ciliary function in the respiratory tract are sparse and contradictory. The purpose of the present study was to clarify the effects of LTD4 on mucociliary activity using the tracheal mucosa of two laboratory animal species, guinea-pig and rat, as well as human nasal mucosa. The ciliary beat frequency (CBF) was measured photoelectrically and determined by Fast Fourier Transform computer analysis. Additionally the structure of ciliated epithelia of guinea-pig trachea after LTD4-immersion was investigated using a scanning electron microscope (SEM). In all tissues, LTD4 increased CBF showing a bell-shaped dose–response curve. The maximum effect was 75±30% in guinea-pig at 10⁻⁹mol/l, 119±49% in rat at 10⁻⁷mol/l, and 86±28% at 10⁻⁶mol/l in human tissue. In guinea-pig tracheal mucosa, there was an indication of an increase in the amount of mucus and disorientation of cilia were seen by SEM after immersion in LTD4. These findings suggest that LTD4 stimulates ciliary activity, but impairs the orientation of cilia.

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白三烯D4对人、豚鼠和大鼠呼吸道黏膜纤毛活性的影响

白三烯D4 (LTD4)是哮喘中一种有效的支气管收缩剂和炎症介质。关于LTD4对呼吸道纤毛功能影响的数据很少且相互矛盾。本研究的目的是利用豚鼠和大鼠两种实验动物的气管粘膜以及人鼻粘膜，阐明LTD4对粘膜纤毛活性的影响。用光电测量纤毛跳动频率(CBF)，用快速傅里叶变换计算机分析确定。此外，用扫描电镜观察了ltd4浸泡后豚鼠气管纤毛上皮的结构。在所有组织中，LTD4增加CBF呈钟形剂量-反应曲线。10−9mol/l时，豚鼠的最大作用为75±30%，10−7mol/l时大鼠的最大作用为119±49%，10−6mol/l时人体组织的最大作用为86±28%。在豚鼠气管粘膜中，LTD4浸泡后，扫描电镜显示粘液量增加，纤毛定向障碍。这些结果表明，LTD4刺激纤毛活动，但损害纤毛的定向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pulmonary pharmacology

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