Mixed disulfides from disulfiram inhibit the benzo[a]pyrene induced mutagenesis

Mutation Research/Genetic Toxicology Pub Date : 1996-12-20 DOI:10.1016/S0165-1218(96)90109-3

Byung-Hoon Lee , Sooyong Lee , Yong-Sun Kim , Barbara Bertram , Manfred Wiessler

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引用次数: 5

Abstract

Some mixed disulfides derived from disulfiram and endogenous thiol compounds have been synthesized, biochemically characterized and their potential antigenotoxic effects have been proposed. The present study evaluated the mutagenic and antimutagenic specificities of two mixed disulfides using S. typhimurium reversion assay, namely S-(N,N-diethyldithiocarbamoly)-N acetylcysteine (AC-DDTC) and -L-glutathione (GS-DDTC). The two mixed disulfides were not mutagenic to Salmonella strains TA98 and TA100 in the presence or absence of S9 mixture. The increased number of revertants by benzo[a]pyrene (B(a)P) has been reduced to the control level by the preincubation with AC-DDTC or GS-DDTC. It was not due to the killing effect of B(a)P, mixed disulfides or B(a)P-disulfide mixture. The antimutagenic effect of AC-DDTC was more potent than that of GS-DDTC. These results indicate that AC-DDTC and GS-DDTC may have a role to play in reducing the risk of mutagenic effects of B(a)P.

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来自双硫仑的混合二硫化物抑制苯并[a]芘诱变

一些由二硫醚和内源性硫醇化合物合成的混合二硫化物已被合成，并进行了生物化学表征，并提出了其潜在的抗毒素作用。本研究采用鼠伤寒沙门氏菌还原试验，评价了S-(N,N-二乙基二硫代氨基甲酸乙酯)-N乙酰半胱氨酸(AC-DDTC)和- l -谷胱甘肽(GS-DDTC)两种混合二硫化物的致突变性和抗诱变性。在有无S9混合物的情况下，两种混合二硫化物对沙门氏菌TA98和TA100均无诱变作用。通过与AC-DDTC或GS-DDTC预孵育，苯并[a]芘(B(a)P)增加的还原物数量已降至对照水平。这不是由于B(a)P、混合二硫化物或B(a)P-二硫化物混合物的杀伤作用。AC-DDTC的抗诱变作用强于GS-DDTC。这些结果提示AC-DDTC和GS-DDTC可能具有降低B(a)P致突变风险的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mutation Research/Genetic Toxicology

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