High-Resolution NMR Probe for Experiments at High Pressures

Abstract

A 300 MHz high-resolution, high-pressure NMR probe which operates in the pressure range of 1 bar to 9 kbar at temperatures of −30 to 100°C is described. Specialized novel design features of the probe are discussed and test spectra showing resolution better than 1 Hz (<3.0 × 10⁻⁹) for 8 mm samples are presented. Potential biochemical applications of this probe are illustrated by experiments dealing with the pressure-induced unfolding of hen egg white lysozyme.