A high performance liquid radiochromatographic assay for the simultaneous analysis of iloprost and misoprostol

Prostaglandins Pub Date : 1996-10-01 DOI:10.1016/S0090-6980(96)00087-1

Iolanda M Womack , Arthur S Lee , Burde Kamath , K.C Agrawal , Vimal Kishore

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引用次数: 5

Abstract

A high-performance liquid chromatographic (HPLC) method utilizing ultraviolet absorbance coupled with radioisotope detection was developed for the precise and simultaneous determination of iloprost and misoprostol. This assay allows complete resolution of iloprost diastereoisomers and has a total run time of approximately twenty minutes. Samples were prepared for chromatographic analysis by extracting a mixture of tritiated drugs from rat plasma with acetonitrile. The resulting solutions were chromatographed on a reversed phase Zorbax Rx-C8 column using 0.02M potassium phosphate (pH 3.0), acetonitrile, and methanol (46:30:24, $v v$ ) at a flow rate of 1.7 mL/min. 2-Naphthoic acid was employed as an internal standard. The correlation coefficient for varying concentrations of tritiated iloprost (12.7 Ci/mmol specific activity) from 2.18 ng/mL to 21.8 ng/mL was 0.995, and the correlation coefficient for concentrations of tritiated misoprostol (50 Ci/mmol specific activity) from 0.617 ng/mL to 6.17 ng/mL was 0.993. The high selectivity and sensitivity of this assay make it useful for the simultaneous quantitation of iloprost and misoprostol.

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一种同时分析伊洛前列素和米索前列醇的高效液相放射色谱法

建立了紫外吸收与放射性同位素检测相结合的高效液相色谱(HPLC)方法，可同时测定伊洛前列素和米索前列醇的含量。该分析允许完全分辨伊洛前列素非对映异构体，总运行时间约为20分钟。用乙腈萃取大鼠血浆中氚化药物的混合物，制备用于色谱分析的样品。得到的溶液在反相Zorbax Rx-C8柱上以0.02M磷酸钾(pH 3.0)、乙腈和甲醇(46:30:24,vv)进行色谱，流速为1.7 mL/min。以2-萘酸为内标。氚化伊洛前列素(12.7 Ci/mmol比活性)浓度在2.18 ~ 21.8 ng/mL范围内的相关系数为0.995，氚化米索前列醇(50 Ci/mmol比活性)浓度在0.617 ~ 6.17 ng/mL范围内的相关系数为0.993。该方法具有高选择性和高灵敏度，可用于同时测定伊洛前列素和米索前列醇的含量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Prostaglandins

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