Altered prostanoid production by cumulus-oocyte complexes in a rat model of non-insulin-dependent diabetes mellitus

Prostaglandins Pub Date : 1996-09-01 DOI:10.1016/S0090-6980(96)00098-6

A. Jawerbaum, E.T. Gonzalez, A. Faletti, V. Novaro, A. Vitullo, M.A.F. Gimeno

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引用次数: 11

Abstract

Ovulation, oocyte maturation and PGE and PGF_2α production by oocyte-cumulus complexes were evaluated in rats with non-insulin-dependent diabetes induced by neonatal streptozotocin. Diabetic rats had normal estrous cycles, but ovulated a lower number of oocytes at estrus. When oocytes from control and diabetic rats obtained at proestrus were matured “in vitro” during 1, 2 or 4 hours (hr) of culture, differences were not found in the percent of germinal vesicle breakdown between both experimental groups. PGE and PGF_2α accumulation was higher in ovulated oocyte-cumulus complexes when compared to immature or “in vitro”-matured oocyte-cumulus complexes in both normal and diabetic rats. When control and diabetic rats are compared, more PGE and PGF_2α accumulation was observed in immature, “in vitro”-matured and in ovulated oocytecumulus complexes.

A lower number of oocytes ovulated and increased oocyte-cumulus complexes prostaglandin production has been observed in this mildly diabetic experimental model. These abnormalities are similar to those previously found when 10 day embryos were evaluated in noninsulin-dependent diabetic rats.

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非胰岛素依赖型糖尿病大鼠模型中卵母细胞复合物改变前列腺素生成

研究了新生儿链脲佐菌素诱导的非胰岛素依赖型糖尿病大鼠的排卵、卵母细胞成熟和卵丘复合物产生PGE和PGF2α的情况。糖尿病大鼠发情周期正常，但发情时卵母细胞数量减少。当对照和糖尿病大鼠在发情前获得的卵母细胞在体外培养1、2或4小时时成熟时，两个实验组之间的生发囊泡破裂百分比没有差异。与未成熟或体外成熟的卵丘复合物相比，正常大鼠和糖尿病大鼠的排卵卵丘复合物中PGE和PGF2α的积累更高。与对照组和糖尿病大鼠相比，未成熟大鼠、体外成熟大鼠和排卵卵丘复合物中PGE和PGF2α的积累更多。在轻度糖尿病实验模型中，观察到排卵的卵母细胞数量减少，卵丘复合物前列腺素的产生增加。这些异常与先前在非胰岛素依赖性糖尿病大鼠中评估10天胚胎时发现的异常相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Prostaglandins

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