[13-week subchronic oral toxicity study of isopropyl p-hydroxybenzoate in F344 rats].

H Onodera, K Mitsumori, K Yasuhara, T Shimo, N Kurokawa, M Takahashi
{"title":"[13-week subchronic oral toxicity study of isopropyl p-hydroxybenzoate in F344 rats].","authors":"H Onodera,&nbsp;K Mitsumori,&nbsp;K Yasuhara,&nbsp;T Shimo,&nbsp;N Kurokawa,&nbsp;M Takahashi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A 13-week subchronic oral toxicity study of isopropyl p-hydroxybenzoate was performed in both sexes of F344 rats. CRF-1 diet containing 0, 0.25, 1.25, 2.5 or 5% isopropyl p-hydroxybenzoate was fed to 5 randomly constituted groups of animals, each consisting of 10 males and 10 females, to determine appropriate dose levels for a subsequent 2-year carcinogenicity study. No animals died during the administration period. Significant suppression of body weight gain was observed in males of the 2.5% and 5% isopropyl p-hydroxybenzoate groups, and in females of the groups treated with 1.25% or above as compared with the control group. Serum biochemistry evaluations revealed increases in gamma-GTP and total cholesterol in male groups treated with 2.5% or more and increases in gamma-GTP, ALP and BUN in female groups treated with 1.25% or more, as compared to the controls. Histopathologically, centrilobular hepatocellular swelling was observed in males treated with 2.5% or more and in females of the 5% group. In the affected populations, hepatocytes filled with small vacuoles, possibly of lipid native, were sometimes found. An increased severity of intracytoplasmic eosinophilic globule formation in the renal proximal tubular epithelia of males of the 5% group was noted. Based on these results, a dietary concentration of 1% in males or 0.5% in females was concluded to be a suitable maximum tolerable dose (MTD) of this chemical for a 2-year carcinogenicity study in rats.</p>","PeriodicalId":11656,"journal":{"name":"Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences","volume":" 112","pages":"82-8"},"PeriodicalIF":0.0000,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

A 13-week subchronic oral toxicity study of isopropyl p-hydroxybenzoate was performed in both sexes of F344 rats. CRF-1 diet containing 0, 0.25, 1.25, 2.5 or 5% isopropyl p-hydroxybenzoate was fed to 5 randomly constituted groups of animals, each consisting of 10 males and 10 females, to determine appropriate dose levels for a subsequent 2-year carcinogenicity study. No animals died during the administration period. Significant suppression of body weight gain was observed in males of the 2.5% and 5% isopropyl p-hydroxybenzoate groups, and in females of the groups treated with 1.25% or above as compared with the control group. Serum biochemistry evaluations revealed increases in gamma-GTP and total cholesterol in male groups treated with 2.5% or more and increases in gamma-GTP, ALP and BUN in female groups treated with 1.25% or more, as compared to the controls. Histopathologically, centrilobular hepatocellular swelling was observed in males treated with 2.5% or more and in females of the 5% group. In the affected populations, hepatocytes filled with small vacuoles, possibly of lipid native, were sometimes found. An increased severity of intracytoplasmic eosinophilic globule formation in the renal proximal tubular epithelia of males of the 5% group was noted. Based on these results, a dietary concentration of 1% in males or 0.5% in females was concluded to be a suitable maximum tolerable dose (MTD) of this chemical for a 2-year carcinogenicity study in rats.

[对羟基苯甲酸异丙酯对F344大鼠13周亚慢性口服毒性研究]。
对羟基苯甲酸异丙酯对F344大鼠进行了为期13周的亚慢性口服毒性研究。将含有0、0.25、1.25、2.5或5%对羟基苯甲酸异丙酯的CRF-1饲粮随机饲喂5组动物,每组10只雄性和10只雌性,以确定随后2年致癌性研究的适当剂量水平。管理期间无动物死亡。2.5%和5%对羟基苯甲酸异丙酯组的雄性体重增加明显受到抑制,1.25%或更高剂量组的雌性与对照组相比体重增加明显受到抑制。血清生化评估显示,与对照组相比,治疗剂量在2.5%或以上的男性组γ - gtp和总胆固醇升高,治疗剂量在1.25%或以上的女性组γ - gtp、ALP和BUN升高。组织病理学上,2.5%及以上剂量组的男性和5%剂量组的女性出现小叶中心肝细胞肿胀。在受影响的人群中,有时发现肝细胞充满小液泡,可能是脂质原生的。注意到5%组男性肾近端小管上皮细胞浆内嗜酸性粒细胞形成的严重程度增加。根据这些结果,在对大鼠进行为期2年的致癌性研究时,得出结论,该化学品的膳食浓度为男性1%或女性0.5%是合适的最大耐受剂量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信