[13-week subchronic toxicity study of L-histidine monohydrochloride in F344 rats].

S Ikezaki, A Nishikawa, F Furukawa, T Imazawa, T Enami, M Mitsui, M Takahashi
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引用次数: 0

Abstract

A 13-week subchronic toxicity study of L-histidine monohydrochloride was performed in male and female F344 rats at dose levels of 0, 0.31, 0.62, 1.25, 2.5 and 5.0% in the diet, to determine the maximum tolerable dose (MTD) for subsequent investigation of carcinogenicity. Rats were randomly allocated to 6 groups, each consisting of 10 males and 10 females. No animals died during the administration period. Suppression of body weight gain and decrease in food consumption were observed in males of the 5.0% group along with hematological examination as revealed by increases in hemoglobin volume and hematocrit. Serum biochemical examination demonstrated increased levels of BUN and creatinine in females of the 5.0% group, and increased level of BUN in females of the 1.25% group. Histopathologically, sperm granulomas in the epididymis were found in half of the 5.0% group males. Based on the results of the present study, it was concluded that the MTD of L-histidine monohydrochloride is 2.5% in diet, because the dietary dose level of 5.0% proved to exert significant toxicity reflected in suppression of body weight gain and formation of sperm granulomas.

[单盐酸l -组氨酸对F344大鼠13周亚慢性毒性研究]。
本研究采用0、0.31、0.62、1.25、2.5和5.0%的剂量对F344大鼠进行了为期13周的亚慢性毒性研究,以确定其最大耐受剂量(MTD),为后续的致癌性研究提供依据。大鼠随机分为6组,每组雄性10只,雌性10只。管理期间无动物死亡。在5.0%组的男性中观察到体重增加的抑制和食物消耗的减少,同时血液检查显示血红蛋白体积和红细胞压积的增加。血清生化检查显示,5.0%组女性BUN和肌酐水平升高,1.25%组女性BUN水平升高。在组织病理学上,5.0%的男性中有一半在附睾发现精子肉芽肿。根据本研究结果,单盐酸l -组氨酸在饲料中的MTD为2.5%,因为饲料剂量水平为5.0%证明具有显著的毒性,体现在抑制体重增加和精子肉芽肿的形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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