A canine coronary artery thrombosis model: application of photochemically induced thrombosis.

Archives internationales de pharmacodynamie et de therapie Pub Date : 1995-07-01

K Umemura, M Ishiye, S Araki, M Nakashima

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Abstract

A simple and reproducible canine model of platelet-rich coronary artery thrombosis, suitable for the evaluation of antithrombotic drugs, has been developed. In this model, thrombus was initiated in the left arterial descending coronary artery by means of transmural green light (540 nm) in the presence of i.v. administered rose bengal (a photosensitizer dye). The photochemical reaction between green light and rose bengal causes endothelial injury which is followed by platelet adhesion aggregation and formation of an occlusive platelet-rich thrombus at the site of the photochemical reaction. In 9 out of 12 animals, occlusion of the coronary artery occurred within 60 min after irradiation with green light and infusion of rose bengal. Infusion of tissue-type plasminogen activator (50,000 IU/kg) produced thrombolysis in 6 out of 9 animals within 60 min. Reocclusion was observed in 4 of these 6 animals. Photochemically induced thrombosis in the canine coronary artery provides a simple and platelet-rich thrombosis model suitable for the evaluation of antithrombotic and thrombolytic preparations.

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犬冠状动脉血栓形成模型:光化学诱导血栓形成的应用。

建立了一种简单、可重复的犬富血小板冠状动脉血栓形成模型，适用于抗血栓药物的评价。在这个模型中，在静脉注射玫瑰红(一种光敏剂染料)的情况下，通过透壁绿光(540 nm)在左动脉冠状动脉降支中引发血栓。绿光和玫瑰红之间的光化学反应引起内皮损伤，随后是血小板粘附聚集和在光化学反应部位形成闭塞的富含血小板的血栓。12只动物中有9只在绿光照射后60分钟内发生冠状动脉闭塞。输注组织型纤溶酶原激活剂(50,000 IU/kg)在60分钟内使9只动物中的6只发生了溶栓。这6只动物中有4只出现了再闭塞。光化学诱导的犬冠状动脉血栓形成提供了一种简单且富含血小板的血栓形成模型，适用于抗栓和溶栓制剂的评价。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Archives internationales de pharmacodynamie et de therapie

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