Dexamethasone stimulation of rat insulin-like growth factor binding protein-1 (IGFBP-1) promoter activity involves the interaction of multiple transcription factors

Progress in growth factor research Pub Date : 1995-01-01 DOI:10.1016/0955-2235(95)00021-6

Dae-Shik Suh, Yuehua Zhou, Guck T. Ooi, Matthew M. Rechler

{"title":"Dexamethasone stimulation of rat insulin-like growth factor binding protein-1 (IGFBP-1) promoter activity involves the interaction of multiple transcription factors","authors":"Dae-Shik Suh, Yuehua Zhou, Guck T. Ooi, Matthew M. Rechler","doi":"10.1016/0955-2235(95)00021-6","DOIUrl":null,"url":null,"abstract":"<div><p>Using an improved procedure for transient transfection of H4-II-E rat hepatoma cells, we characterized the <em>cis</em> elements in the proximal promoter of the rat insulin-like growth factor binding protein-1 (rat IGFBP-1) gene that are required for basal (unstimulated) and dexamethasone-stimulated promoter activity. Three sites are required for optimal basal promoter activity: an AP-2 site (nt −286 to −293), the M4 region of the insulin response element (nt −108 to −99), and a hepatocyte nuclear factor-1 (HNF-1) site (nt −62 to −50). In addition to the glucocorticoid response element (nt −91 to −77), participation of two of three accessory sites is required for optimal stimulation by dexamethasone: the M4 and HNF-1 sites, and a third site located between nt −252 and −236. Further study will focus on how the interactions of tissue-specific and hormonally-responsive transcription factors are integrated.</p></div>","PeriodicalId":77335,"journal":{"name":"Progress in growth factor research","volume":"6 2","pages":"Pages 131-140"},"PeriodicalIF":0.0000,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0955-2235(95)00021-6","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Progress in growth factor research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0955223595000216","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 6

Abstract

Using an improved procedure for transient transfection of H4-II-E rat hepatoma cells, we characterized the cis elements in the proximal promoter of the rat insulin-like growth factor binding protein-1 (rat IGFBP-1) gene that are required for basal (unstimulated) and dexamethasone-stimulated promoter activity. Three sites are required for optimal basal promoter activity: an AP-2 site (nt −286 to −293), the M4 region of the insulin response element (nt −108 to −99), and a hepatocyte nuclear factor-1 (HNF-1) site (nt −62 to −50). In addition to the glucocorticoid response element (nt −91 to −77), participation of two of three accessory sites is required for optimal stimulation by dexamethasone: the M4 and HNF-1 sites, and a third site located between nt −252 and −236. Further study will focus on how the interactions of tissue-specific and hormonally-responsive transcription factors are integrated.

查看原文本刊更多论文

地塞米松刺激大鼠胰岛素样生长因子结合蛋白-1 (IGFBP-1)启动子活性涉及多种转录因子的相互作用

利用一种改进的瞬时转染H4-II-E大鼠肝癌细胞的方法，我们鉴定了大鼠胰岛素样生长因子结合蛋白-1(大鼠IGFBP-1)基因近端启动子中的顺式元件，这些元件是基础(未刺激)和地塞米松刺激启动子活性所必需的。最佳基础启动子活性需要三个位点:AP-2位点(nt - 286至- 293)，胰岛素反应元件的M4区域(nt - 108至- 99)和肝细胞核因子-1 (HNF-1)位点(nt - 62至- 50)。除了糖皮质激素反应元件(nt - 91至- 77)外，地塞米松的最佳刺激还需要三个辅助位点中的两个参与:M4和HNF-1位点，以及位于nt - 252至- 236之间的第三个位点。进一步的研究将集中在组织特异性和激素反应性转录因子的相互作用如何整合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Progress in growth factor research

自引率

0.00%

发文量