Pharmacological studies on mechanisms involved in Ca2+ oscillations in rat megakaryocytes

European Journal of Pharmacology: Molecular Pharmacology Pub Date : 1995-11-30 DOI:10.1016/0922-4106(95)90080-2

Chikako Uneyama , Hisayuki Uneyama , Michihito Takahashi , Norio Akaike

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引用次数: 3

Abstract

Extracellular application of ATP evoked the oscillatory K⁺ currents (I_KCa) reflecting oscillation in cytoplasmic Ca²⁺ concentration ([Ca²⁺]_i) of megakaryocyte isolated from rat bone marrow. We have reported that the [Ca²⁺]_i oscillation was regulated by intracellular Ca²⁺-pumping activity (Uneyama, H., C. Uneyama and N. Akaike, 1993, J. Biol. Chem. 268, 168). Here we found that the Ca²⁺ pump of the megakaryocyte could be divided into at least two classes according to the sensitivity to phosphorylation-modulating drugs. The effects of protein kinase C and cyclic AMP-dependent protein kinase are complementary, and the effect of Ca²⁺/calmodulin is independent of the above two kinases. In addition, this is the first report concerning the physiological regulation of Ca²⁺-ATPase in living cells.

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大鼠巨核细胞Ca2+振荡机制的药理学研究

细胞外应用ATP引起振荡K+电流(IKCa)，反映了从大鼠骨髓分离的巨核细胞胞质Ca2+浓度([Ca2+]i)的振荡。我们已经报道了细胞内Ca2+泵送活动调节[Ca2+]i振荡(Uneyama, H.， C. Uneyama and N. Akaike, 1993, J. Biol.)。化学。268,168)。这里我们发现，根据对磷酸化调节药物的敏感性，巨核细胞的Ca2+泵可以分为至少两类。蛋白激酶C和环amp依赖性蛋白激酶的作用是互补的，Ca2+/钙调蛋白的作用独立于上述两种激酶。此外，这是关于Ca2+- atp酶在活细胞中的生理调节的第一篇报道。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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European Journal of Pharmacology: Molecular Pharmacology

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