Immunohistochemical distribution of S-100 protein and type IV collagen in human embryonic and fetal sympathetic neuroblasts.

The Histochemical Journal Pub Date : 1995-09-01
G Magro, S Grasso, C Emmanuele
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Abstract

The expression and distribution of S-100 protein and type IV collagen was studied immunohistochemically in sympathetic neuroblasts from the paravertebral region to the adrenal glands in human embryos and fetuses ranging from 7 to 12 weeks gestational age. From 7 weeks gestational age, S-100 protein was detected in round or oval cells mingling with sympathetic neuroblasts, and in spindle-shaped cells forming a continuous layer around them. The latter S-100 protein-positive cells were found in contact with the Schwann cells of nerve fibres entering the groups of sympathetic neuroblasts. Staining for type IV collagen showed that all groups of sympathetic neuroblasts were surrounded by a continuous basement membrane. By examining serial sections stained for type IV collagen and S-100 protein, a continuous basement membrane was found along the distribution pattern of the peripheral S-100 protein-positive spindle cells. The morphology of these cells, and their relationships with Schwann cells and with the basement membrane of the sympathetic neuroblasts, indicated that they were Schwann-like cells probably capable of synthesizing a continuous basement membrane separating the neuroblasts from the adjacent tissues. In contrast, the round or oval S-100 protein-positive cells, in contact with the sympathetic neuroblasts and not associated with nerve fibres, were considered as sustentacular or sustentacular precursor cells. At week 7 gestational age, the peri-adrenal sympathetic neuroblasts and their sustentacular and Schwann-like cells started to invade the adrenal glands and mingled with the adrenal cortical cells. These findings suggest the extra-adrenal origin of the sustentacular cells in embryonic and fetal adrenal glands.

S-100蛋白和IV型胶原在人胚胎和胎儿交感神经母细胞中的免疫组织化学分布。
采用免疫组织化学方法研究了S-100蛋白和IV型胶原蛋白在7 ~ 12孕周人胚胎和胎儿椎旁至肾上腺交感神经母细胞中的表达和分布。从孕7周开始,S-100蛋白在与交感神经母细胞混合的圆形或椭圆形细胞以及在其周围形成连续层的纺锤形细胞中检测到。后一种S-100蛋白阳性细胞与神经纤维的雪旺细胞接触,进入交感神经母细胞群。IV型胶原染色显示,所有组交感神经母细胞均被连续的基底膜包围。通过检查IV型胶原和S-100蛋白染色的连续切片,发现沿周围S-100蛋白阳性梭形细胞的分布模式有连续的基底膜。这些细胞的形态以及它们与雪旺细胞和交感神经母细胞基底膜的关系表明,它们是雪旺样细胞,可能能够合成一个连续的基底膜,将神经母细胞与邻近组织分开。相反,圆形或椭圆形的S-100蛋白阳性细胞,与交感神经母细胞接触,与神经纤维无关,被认为是支撑带或支撑带前体细胞。孕7周时,肾上腺周围交感神经母细胞及其支撑带细胞和雪旺样细胞开始侵入肾上腺,并与肾上腺皮质细胞混合。这些发现提示了支持细胞在胚胎和胎儿肾上腺的肾上腺外起源。
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