A simple field method for the purification of Onchocerca ochengi microfilariae from a mixed Onchocerca infection in cattle.

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1995-09-01

H E Hagen, S L Kläger, P J Ham

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Abstract

Onchocerca ochengi, a bovine parasite, is a suitable model for research on human River Blindness. However, the microfilariae are normally found concomitantly with at least one of the other three bovine Onchocerca species O. dukei, O. gutturosa and O. armillata causing difficulties for the work on the microfilariae. We describe a simple and field applicable method for the separation of living O. ochengi microfilariae from the other Onchocerca species using Sephadex G-25 columns. Elution of mixed populations resulted in the passage of O. gutturosa and/or O. dukei in the initial 1 ml fraction with O. ochengi eluting as an almost 100% pure species in the 4th and 5th fractions.

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从牛混合盘尾丝虫感染中纯化奥氏盘尾丝虫的简易现场方法。

牛盘尾丝虫是研究人类河盲症的合适模型。然而，微丝虫通常与其他三种牛盘尾丝虫中的至少一种同时存在，这给微丝虫的研究带来了困难。采用Sephadex G-25色谱柱，建立了一种简便、现场适用的方法，从盘尾丝虫中分离出活的ochengi微丝虫。混合群体的洗脱结果表明，在最初的1 ml馏分中，古特罗萨和/或杜卡伊的传代率几乎为100%，而在第4和第5馏分中，奥chengi的传代率几乎为100%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)

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