S S Matsuyama, D T Yamaguchi, Y Vergara, L F Jarvik
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引用次数: 10
Abstract
Potassium (K+) channel dysfunction in fibroblasts was recently proposed as a potential diagnostic marker for Alzheimer disease (AD). We utilized a microspectrofluorometric method with Fura-2AM to measure intracellular free calcium ([Ca2+]i) following depolarization with the K+ channel blocker tetraethylammonium (TEA) in seven AD and seven control fibroblast cultures. Contrary to our expectation, 43% of the AD and 36% of the control fibroblast plated coverglasses responded with an increase in [Ca2+]i on addition of 100 mM TEA. The data suggest that the TEA-elicited [Ca2+]i response is not a useful AD screening test.
最近，成纤维细胞中的钾离子通道功能障碍被认为是阿尔茨海默病(AD)的潜在诊断标志物。在7个AD和7个对照成纤维细胞培养中，我们利用Fura-2AM微荧光光谱法测量了K+通道阻滞剂四乙基铵(TEA)去极化后的细胞内游离钙([Ca2+]i)。与我们的预期相反，43%的AD和36%的对照成纤维细胞镀盖玻璃在添加100 mM TEA时反应增加[Ca2+]i。数据表明，tea诱导的[Ca2+]i反应不是一种有用的AD筛选试验。
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