{"title":"Expression of the DNA repair gene XRCC1 in baboon tissues","authors":"Zi-Qiang Zhou, Christi A. Walter","doi":"10.1016/0165-7992(95)00053-4","DOIUrl":null,"url":null,"abstract":"<div><p><em>XRCC1</em> is a DNA repair gene involved in rejoining DNA strand-breaks. We used baboon as an animal model to determine the levels of <em>XRCC1</em> gene expression in different tisues. Baboons were selected because they are evolutionarily closely related to humans. A single 2.2 kb transcript was detected in all tissues tested by northern blot analysis, with variations in levels of expression among tissues. The expression levels of <em>XRCC1</em> were measured by quantitative RNase protection assays. <em>XRCC1</em> mRNA levels were significantly higher in testis than those other tissues. A mean value of 24.6 × 10<sup>5</sup> <em>XRCC1</em> transcripts per μg DNA was found in testis, while 10.5 × 10<sup>5</sup> in ovary, 9.8 × 10<sup>5</sup> in brain, 8.5 × 10<sup>5</sup> in liver, 6.8 × 10<sup>5</sup> in kidney, 6.5 × 10<sup>5</sup> in heart, 6.4 × 10<sup>5</sup> in lymph nodes, 6.0 × 10<sup>5</sup> in lung and 4.9 × 10<sup>5</sup> in spleen were found.</p></div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"348 3","pages":"Pages 111-116"},"PeriodicalIF":0.0000,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)00053-4","citationCount":"35","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research Letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0165799295000534","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 35
Abstract
XRCC1 is a DNA repair gene involved in rejoining DNA strand-breaks. We used baboon as an animal model to determine the levels of XRCC1 gene expression in different tisues. Baboons were selected because they are evolutionarily closely related to humans. A single 2.2 kb transcript was detected in all tissues tested by northern blot analysis, with variations in levels of expression among tissues. The expression levels of XRCC1 were measured by quantitative RNase protection assays. XRCC1 mRNA levels were significantly higher in testis than those other tissues. A mean value of 24.6 × 105XRCC1 transcripts per μg DNA was found in testis, while 10.5 × 105 in ovary, 9.8 × 105 in brain, 8.5 × 105 in liver, 6.8 × 105 in kidney, 6.5 × 105 in heart, 6.4 × 105 in lymph nodes, 6.0 × 105 in lung and 4.9 × 105 in spleen were found.
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