[Histoautoradiographic and morphometric analysis of myocardial tissue in optimal regimen of hyperbaric oxygenation].

Abstract

The experiment on 90 rabbits and 30 rats has been conducted to compare the effect of different regimes of hyperbaric oxygenation (HBO) (pressure of oxygen: 3 ata, 2 ata, oxygen-air mixture: 3 ata and 1 ata, respectively) on the morphofunctional state of intact and ischemic myocardium with experimental infarction. The histoautoradiographic method using 3H-thymidine and 3H-uridine, the precursors of nucleic acids' synthesis, proved a good criterion of viability and activity of the regenerative processes in cardiomyocytes of perinfarction and necrosis-distant zones of the heart. The best effect has been obtained when using HBO regime with oxygen pressure 2 ata, the highest damaging effect--oxygen (3 ata)-air (1 ata) mixture. The use of HBO has prevented the development of necrosis foci and dystrophic changes in the auricles.