{"title":"Calcium induced modulation of peripheral-type benzodiazepine receptors in rat kidney membranes.","authors":"D J Calvo, J H Medina","doi":"10.3109/10799899309073704","DOIUrl":null,"url":null,"abstract":"<p><p>The effects of Ca2+ ions on 3H-RO 5-4864 binding to the peripheral benzodiazepine receptor were examined. Preincubation of rat kidney membranes with Ca2+ at 37 degrees C produced a dose-dependent inhibition of 3H-RO 5-4864 binding. No inhibition was observed in membranes preincubated at 0 degrees C. The effect of Ca2+ was competitive in nature and was fully reversed by the addition of EGTA. At 1 mM, the maximal effect was achieved with CaCl2, whereas CoCl2 and CdCl2 had lesser effects. No other divalent cation salts examined decreased 3H-RO 5-4864 binding to rat kidney membranes. Collectively, these data demonstrate that the affinity of 3H-RO 5-4864 binding to rat kidney membranes is regulated by Ca2+ and suggest the presence of cation recognition binding sites coupled to the peripheral benzodiazepine receptor.</p>","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"13 6","pages":"975-87"},"PeriodicalIF":0.0000,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899309073704","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of receptor research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10799899309073704","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The effects of Ca2+ ions on 3H-RO 5-4864 binding to the peripheral benzodiazepine receptor were examined. Preincubation of rat kidney membranes with Ca2+ at 37 degrees C produced a dose-dependent inhibition of 3H-RO 5-4864 binding. No inhibition was observed in membranes preincubated at 0 degrees C. The effect of Ca2+ was competitive in nature and was fully reversed by the addition of EGTA. At 1 mM, the maximal effect was achieved with CaCl2, whereas CoCl2 and CdCl2 had lesser effects. No other divalent cation salts examined decreased 3H-RO 5-4864 binding to rat kidney membranes. Collectively, these data demonstrate that the affinity of 3H-RO 5-4864 binding to rat kidney membranes is regulated by Ca2+ and suggest the presence of cation recognition binding sites coupled to the peripheral benzodiazepine receptor.
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