Uncoupling of mitochondrial oxidative phosphorylation abolishes the stimulatory action of insulin on the binding of glycolytic enzymes to muscle cytoskeleton.

T Livnat, M Chen-Zion, R Beitner
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引用次数: 9

Abstract

1. We show here that treatment of diaphragm muscle with 2,4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation, abolished the stimulatory action of insulin on binding of the glycolytic enzymes, phosphofructokinase (PFK) and aldolase, to muscle cytoskeleton. This effect was demonstrated with low concentration of DNP, which caused only a small decrease in ATP and did not affect the basic levels of cytoskeleton-bound glycolytic enzymes. 2. Higher concentrations of DNP, which induced a drastic decline in ATP content, caused a decrease in cytoskeleton-bound glycolytic enzymes and damage to myofibrils. 3. These results suggest that mitochondrial ATP is required for both the preservation of the basal levels of cytoskeleton-bound glycolytic enzymes and cell structure, as well as for the expression of the stimulatory action of insulin on glycolytic enzymes' binding to muscle cytoskeleton.

线粒体氧化磷酸化的解偶联消除了胰岛素对糖酵解酶与肌肉细胞骨架结合的刺激作用。
1. 我们在这里表明,用2,4-二硝基苯酚(DNP)处理膈肌,一种氧化磷酸化解耦剂,消除了胰岛素对糖酵解酶,磷酸果糖激酶(PFK)和醛缩酶结合肌肉细胞骨架的刺激作用。低浓度的DNP证明了这种作用,它只引起ATP的少量减少,并且不影响细胞骨架结合的糖酵解酶的基本水平。2. 较高浓度的DNP诱导ATP含量急剧下降,导致细胞骨架结合糖酵解酶减少和肌原纤维损伤。3.这些结果表明,线粒体ATP对于维持细胞骨架结合的糖酵解酶的基础水平和细胞结构,以及胰岛素对糖酵解酶与肌肉细胞骨架结合的刺激作用的表达都是必需的。
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