[Inhibitors of nucleic acid synthesis as a means of identifying the forms of DNA-dependent DNA polymerases in Acholeplasma laidlawii PG-8 and of determining their functions].

Mikrobiologicheskii zhurnal Pub Date : 1993-05-01
I G Skripal', S V Bezuglyĭ, V V Babichev
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引用次数: 0

Abstract

Antibiotics, inhibitors of nucleic acids' synthesis from the group of chromomycins (olivomycin of sodium salt), anthracyclines (carminomycin and doxorubicin) and streptonigrin (bruneomycin) have been studied for their effect on DNA synthesis in vitro performed by DNA polymerases (1st and 2nd forms) of Acholeplasma laidlawii PG-8. It has been stated that olivomycin inhibits the function of both the first and second forms of DNA polymerases in proportion to an increase of the antibiotic concentration in the medium. Carminomycin in the concentration of about 1 microgram/ml almost completely inhibited the activity of both DNA polymerases. However, doxorubicin also belonging to the group of anthracyclins completely inhibited the activity of the first form of DNA polymerase in the concentration of 1 microgram/ml and practically has no effect in the concentration up to 100 micrograms/ml on the activity of the second form possessing 3'-->5'-function. Streptonigrin also proved to be suitable for differentiate the forms of DNA polymerases and to determine their functions. The first form of DNA polymerase with 5'-->3'-polymerase and exonuclease functions was not sensitive by this antibiotic in the concentration of 1000 micrograms/ml, while the activity of the second form of DNA polymerase with 3'-->5'-exonuclease functions was fully inhibited by this concentration of the antibiotic in the medium. The combination of doxorhubicin and streptonigrin in the medium can be used to determine the form of DNA polymerases and to identify their 5'-->3'- or 3'-->5'-exonuclease function and for selectivity inhibition of the function of one or another DNA polymerase in the medium.

[核酸合成抑制剂,作为一种鉴定莱氏体PG-8中DNA依赖的DNA聚合酶形式和确定其功能的手段]。
研究了抗生素、由嗜铬霉素(钠盐橄榄霉素)、蒽环类(卡米霉素和多柔比星)和链霉素(布吕霉素)组成的核酸合成抑制剂对克拉氏等离子体PG-8 DNA聚合酶(1型和2型)体外合成DNA的影响。已经指出,橄榄霉素抑制第一和第二种形式的DNA聚合酶的功能与培养基中抗生素浓度的增加成比例。卡米诺霉素浓度约为1微克/毫升时,几乎完全抑制了两种DNA聚合酶的活性。然而,同样属于蒽环类的阿霉素在浓度为1微克/毫升时,完全抑制了第一种形式的DNA聚合酶的活性,而在浓度高达100微克/毫升时,对具有3'- >5'功能的第二种形式的活性几乎没有影响。链红素也被证明适合于区分DNA聚合酶的形式和确定它们的功能。具有5′->3′-聚合酶和外切酶功能的第一种形式的DNA聚合酶在1000微克/ml浓度下对该抗生素不敏感,而具有3′->5′-外切酶功能的第二种形式的DNA聚合酶在该浓度的培养基中被完全抑制。多柔比星和链霉素在培养基中的组合可用于确定DNA聚合酶的形式和鉴定其5'- >3'或3'- >5'-外切酶的功能,以及选择性抑制培养基中一种或另一种DNA聚合酶的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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