Radical trapping by PBN during reperfusion in rabbit gastric mucosa.

Free radical research communications Pub Date : 1993-01-01 DOI:10.3109/10715769309056s185

M Sonoda, G Asakuno, M Matsuki, A Satomi, K Ishida, Y Sakagishi

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引用次数: 8

Abstract

Oxygen free radicals have been considered as a cause of ischemia-reperfusion injury in several organs, but this injury in a stomach, containing acid, may progress to severe damage. Thus, we examined the effect of ischemia-reperfusion and milk-intake on rabbit gastric mucosa. The gastric mucosal blood flow was increased after milk-intake, and gastric rupture was detected. Superoxide dismutase activity measured by an improved nitroblue tetrazolium reduction method and thiobarbituric acid reactive substances in serum is increased during ischemia-reperfusion and milk-intake. By using alpha-phenyl N-tert-butyl nitrone (PBN) as a spin trap and electron paramagnetic resonance (EPR), we detected lipidic radicals from tissue samples in chloroform-methanol solvent only during reperfusion and milk-intake period; no signal was detected before. The EPR signal of spin adducts obtained in the sample after ischemia-reperfusion and milk-intake would be a mixture of peroxyl and alkoxyl radicals from the analysis of coupling constants.

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PBN在兔胃粘膜再灌注过程中的自由基捕获作用。

氧自由基已被认为是几个器官缺血-再灌注损伤的原因，但这种损伤在胃中，含有酸，可能会发展为严重的损害。因此，我们研究了缺血再灌注和牛奶摄入对兔胃粘膜的影响。牛奶摄入后胃黏膜血流量增加，胃破裂。改良硝基蓝四氮唑还原法测定血清超氧化物歧化酶活性和硫代巴比妥酸反应性物质在缺血再灌注和牛奶摄入时升高。采用α -苯基n -叔丁基硝基(PBN)作为自旋陷阱和电子顺磁共振(EPR)技术，仅在氯仿-甲醇溶剂中再灌注和牛奶摄入期间检测组织样品中的脂质自由基;之前没有检测到信号。从耦合常数的分析来看，样品在缺血再灌注和牛奶摄入后得到的自旋加合物的EPR信号可能是过氧基和烷氧基自由基的混合物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Free radical research communications

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