Salivary gland surface carbohydrate variations in three species of the Anopheles gambiae complex.

H A Mohamed, G A Ingram
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Abstract

Fluorescein isothiocyanate (FITC)-conjugated lectins (agglutinins) were employed as probes to distinguish between the various carbohydrates present on the surface of salivary glands of three species of mosquito of the Anopheles gambiae complex. Of twenty lectins tested, eight (Concanavalin A- Con A, Lathyrus odoratus- LOA, Lens culinaris, Pisum sativum-PSA, Vicia faba- VFA, Triticum vulgaris, Maclura pomifera- MPA and Ulex europaeus) specifically reacted with the salivary gland membrane. Both mannosyl and N-acetylglucosamine moieties were detected in the three Anopheles gambiae sensu stricto strains, the two An. arabiensis strains and a single An. merus strain examined. Variations in the degrees of fluorescent intensities of Con A and MPA in particular suggested interspecies differences in membrane mannosyl and galactosyl residues on the salivary gland lobes of the three mosquito species in this study. Furthermore, intraspecific variations in mannose as indicated by Con A, LOA, PSA and VFA staining were demonstrated between the An. gambiae s.s. strains. The use of either peroxidase-labelled or biotinylated lectins confirmed the binding specificities of the above lectins. The consistent differences observed in lectin binding suggest that variations occur in salivary gland surface carbohydrate residues and that lectins can be used to distinguish between at least some members of the An. gambiae complex.

冈比亚按蚊复合体的三种唾液腺表面碳水化合物的变化。
采用异硫氰酸荧光素(FITC)偶联凝集素(凝集素)作为探针,对冈比亚按蚊(Anopheles gambiae)复合体中3种蚊子唾液腺表面的碳水化合物进行了区分。在测试的20种凝集素中,有8种凝集素与唾液腺膜发生特异性反应,分别为:刀豆素A- Con A、臭草素- LOA、烹饪Lens、豌豆素- psa、蚕豆素- VFA、普通小麦素、pomifera- MPA和Ulex europaeus。3株冈比亚按蚊中均检测到甘露糖基和n -乙酰氨基葡萄糖。arabiensis菌株和单一的An。检查颈部应变。特别是Con A和MPA荧光强度的变化表明,本研究中三种蚊子唾液腺叶上的甘露糖基和半乳糖基膜残留在种间存在差异。此外,通过Con A、LOA、PSA和VFA染色,证实了甘露糖的种内变化。冈比亚菌株。使用过氧化物酶标记的或生物素化的凝集素证实了上述凝集素的结合特异性。在凝集素结合中观察到的一致差异表明,唾液腺表面碳水化合物残基发生了变化,凝集素可以用来区分至少一些成员。冈比亚按蚊的复杂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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