Ultrastructural investigation of DNA in megakaryoblastic leukemia by using osmium-ammine-B: comparison with several types of leukemia.

Y Ohwada, M Eguchi
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Abstract

Using DNA staining with highly stable osmium-ammine-B, the blasts of AMKL were observed under an electron microscope, in comparison with AML, ALL, CML-MK crisis and TMD. The DNA within the nucleus of the megakaryoblasts was observed as a high electron-density substance and tended to be uniformly dispersed within the nucleus. DNA, associated with nucleoli, could be roughly divided in four types based on the presence or absence of peri-nucleolar clumps and intra-nucleolar clumps. In cases of AMKL, we often observed a type that did not have peri-nucleolar DNA clumps but did have DNA flecks within the nucleoli. By analytical evaluation there were no differences among cells from several subtypes of megakaryocytic leukemia, such as AMKL in children with Down's syndrome, AMKL in children without Down's syndrome, AMKL in adults, and CML-MK crisis. The DNA distribution of TMD blasts, which were self-limited and not malignant, resembled that of AMKL blasts.

锇胺b对巨核母细胞白血病DNA超微结构的研究:与几种类型白血病的比较。
采用高稳定的锇胺b DNA染色,电镜下观察AMKL细胞的形成,并与AML、ALL、CML-MK危象和TMD进行比较。巨核母细胞细胞核内的DNA是一种高电子密度的物质,并倾向于均匀分散在细胞核内。与核仁相关的DNA,根据核仁周围团块和核仁内团块的存在与否大致可分为四种类型。在AMKL病例中,我们经常观察到一种类型没有核仁周围DNA团块,但核仁内确实有DNA斑点。经分析评估,不同亚型巨核细胞白血病(如唐氏综合征患儿AMKL、非唐氏综合征患儿AMKL、成人AMKL、CML-MK危象)细胞间无差异。TMD细胞的DNA分布与AMKL细胞相似,具有自限性和非恶性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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