Separation of rare cell subpopulations with the aid of biotin-labelled ligands.

M Steinitz, A Livoff, S Tamir, T Brenner
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引用次数: 1

Abstract

A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are first labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10(-4). The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).

利用生物素标记配体分离稀有细胞亚群。
描述了一种选择表面标记阳性细胞的通用方法。这些细胞与过量的表面标记阴性细胞混合,首先用特定的生物素化配体标记,然后用单克隆抗生物素包被珠分离。该方法能够选择和分离频率低至10(-4)的细胞。配体可以是抗原(用于选择很少产生抗体的细胞),抗体(用于选择表面抗原阳性细胞)或其他分子(用于选择特定的受体阳性细胞)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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