Regional differences in glucose transport in the mouse hippocampus.

The Histochemical Journal Pub Date : 1994-03-01
M Shimada, S Kawamoto, Y Hirose, M Nakanishi, H Watanabe, M Watanabe
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Abstract

In order to observe glucose transport into the brain, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (NBDG), a non-metabolizable and fluorescent glucose analogue, was injected intravenously into mice. After ascertaining that this glucose analogue is non-metabolizable in the brain, the NBDG contents in the blood and brain were measured quantitatively by spectrofluorimetry at 0, 0.5, 2, 5, 10 and 30 min after intravenous injection. The NBDG content in the blood decreased markedly with time, whereas in the brain it rapidly decreased, then gradually increased after 2 min. Glucose transport into the hippocampus was observed with a confocal laser scanning microscope. At 0.5 min, NBGD was seen to be highly concentrated on the vascular wall. Using the confocal mode, it was found that the fluorescence was unevenly distributed on the microvessel wall, suggesting local differences of glucose transport in the vascular wall. At 5 min, the fluoresent intensity of the vascular wall was markedly decreased, whereas relatively intense fluorescence was observed in the cerebral parenchyma of the stratum lacunosum-moleculare and stratum pyramidale of Ca3. At 10 min, a weak fluoresence was diffusely distributed in the hippocampus. As to the localization of NBDG in the brain, capillary endothelium (luminal and abluminal membrane), basement membrane, and the feet of the astrocytes are discussed.

小鼠海马中葡萄糖转运的区域差异。
为了观察葡萄糖在脑内的转运,将非代谢的荧光葡萄糖类似物6-[N-(7-硝基苯-2-氧-1,3-二唑-4-基)氨基]-6-脱氧葡萄糖(NBDG)静脉注射到小鼠体内。在确定该葡萄糖类似物在脑内不可代谢后,在静脉注射后0、0.5、2、5、10和30 min,用荧光光度法定量测定血液和脑内NBDG的含量。随着时间的延长,血中NBDG含量明显下降,脑中NBDG含量迅速下降,2 min后逐渐升高。共聚焦激光扫描显微镜观察葡萄糖向海马的转运。0.5 min时,NBGD高度集中在血管壁上。利用共聚焦模式发现,荧光在微血管壁上分布不均匀,提示葡萄糖在血管壁上转运存在局部差异。5min时,血管壁荧光强度明显减弱,而Ca3的腔隙层-分子层和锥体层的脑实质荧光强度相对较强。10min时,微弱荧光在海马区弥漫性分布。探讨了星形胶质细胞在脑内的定位,包括毛细血管内皮(管腔和腔膜)、基底膜和足部。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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