Chitinolytic properties of Bacillus pabuli K1.

E Frändberg, J Schnürer
{"title":"Chitinolytic properties of Bacillus pabuli K1.","authors":"E Frändberg,&nbsp;J Schnürer","doi":"10.1111/j.1365-2672.1994.tb01641.x","DOIUrl":null,"url":null,"abstract":"<p><p>The chitinolytic properties of Bacillus pabuli K1 isolated from mouldy grain was studied. Chitinase activity was measured as the release of p-nitrophenol from p-nitrophenyl-N,N'-diacetylchitobiose. Influences of substrate concentration and different environmental variables on growth and chitinase activity were determined. The optimum environmental conditions for chitinase production were: 30 degrees C, initial pH 8, initial oxygen 10% and aw > 0.99. Chitinase production was induced when B. pabuli K1 was grown on colloidal chitin. The smallest chito-oligosaccharide able to induce chitinase production was N,N'-diacetylchitobiose, (GlcNAc)2. Production was also induced by (GlcNAc)3 and (GlcNAc)4. When the bacterium was grown on glucose or N-acetylglucosamine, no chitinases were formed. The highest chitinase production observed was obtained with colloidal chitin as substrate. The production of chitinases by B. pabuli K1 growing on chitin was repressed by high levels (0.6%) of glucose. The production was also repressed by 0.6% starch, laminarin and beta-glucan from barley and by glycerol. The addition of pectin and carboxymethyl cellulose increased chitinase production.</p>","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"76 4","pages":"361-7"},"PeriodicalIF":0.0000,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb01641.x","citationCount":"75","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of applied bacteriology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1365-2672.1994.tb01641.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 75

Abstract

The chitinolytic properties of Bacillus pabuli K1 isolated from mouldy grain was studied. Chitinase activity was measured as the release of p-nitrophenol from p-nitrophenyl-N,N'-diacetylchitobiose. Influences of substrate concentration and different environmental variables on growth and chitinase activity were determined. The optimum environmental conditions for chitinase production were: 30 degrees C, initial pH 8, initial oxygen 10% and aw > 0.99. Chitinase production was induced when B. pabuli K1 was grown on colloidal chitin. The smallest chito-oligosaccharide able to induce chitinase production was N,N'-diacetylchitobiose, (GlcNAc)2. Production was also induced by (GlcNAc)3 and (GlcNAc)4. When the bacterium was grown on glucose or N-acetylglucosamine, no chitinases were formed. The highest chitinase production observed was obtained with colloidal chitin as substrate. The production of chitinases by B. pabuli K1 growing on chitin was repressed by high levels (0.6%) of glucose. The production was also repressed by 0.6% starch, laminarin and beta-glucan from barley and by glycerol. The addition of pectin and carboxymethyl cellulose increased chitinase production.

pabuli芽孢杆菌K1的几丁质降解特性。
研究了从霉变谷物中分离得到的pabuli芽孢杆菌K1的几丁质降解特性。通过对硝基-N,N'-二乙酰壳聚糖释放对硝基苯酚来测定几丁质酶的活性。测定底物浓度和不同环境变量对几丁质酶活性和生长的影响。产几丁质酶的最佳环境条件为:30℃,初始pH 8,初始氧10%,aw > 0.99。在胶体几丁质上生长的pabuli K1诱导了几丁质酶的产生。能诱导几丁质酶产生的最小的壳寡糖是N,N'-二乙酰壳寡糖(GlcNAc)2。(GlcNAc)3和(GlcNAc)4也能诱导其产生。当细菌在葡萄糖或n -乙酰氨基葡萄糖上生长时,没有几丁质酶形成。以胶体几丁质为底物的几丁质酶产量最高。生长在几丁质上的B. pabuli K1产生几丁质酶受到高水平(0.6%)葡萄糖的抑制。0.6%的淀粉、大麦中的层粘连蛋白和β -葡聚糖以及甘油也抑制了产量。果胶和羧甲基纤维素的添加增加了几丁质酶的产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信