[The effect of N,N'-bis(decyldimethyl)-1,6-hexane diammonium dibromide on vegetative cells and spores of type A Clostridium perfringens].

Abstract

The author investigated the effect of N,N'-bis(decyldimethyl)-1,6-hexane diammonium dibromide on vegetative cells and spores of Clostridium perfringens type A. The investigated substance caused in concentrations of 1.0-4.0 micrograms.ml-1 escape of K+ from clostridia cells. At higher concentrations-2.0-16.0 micrograms.ml-1-high molecular components of the cytoplasmic material escaped. The author investigated the effect of the mentioned substance on germination of spores of Clostridium perfringens type A, using concentrations of 10, 20 and 40 micrograms.ml-1. The lower concentration of the substance (10 micrograms.ml-1) did not inhibit the onset of germination of spores of the tested strain, however, the post-germination development was retarded, as compared with controls. Germination of spores of Cl. perfringens type A in the presence of higher concentrations of the compound (20 and 40 micrograms.ml-1) was affected already in the initial stage. The germinating spores were most sensitive to the action of the substance when it was added after 60 and 120 min. following activation of spores for germination. The tested substance reduced the thermal resistance of Cl. perfringens type A spores.