Systemic and regional hemodynamic changes during endotoxin or platelet activating factor (PAF)-induced shock in rats.

Circulatory shock Pub Date : 1993-12-01
M F Mulder, A A van Lambalgen, A A van Kraats, P G Scheffer, A A Bouman, G C van den Bos, L G Thijs
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Abstract

To evaluate the role of platelet activating factor (PAF) during endotoxin shock, we compared its effects with those of endotoxin. We measured arterial pressure (MAP), heart rate (HR), cardiac output (CO; thermodilution), arterial lactate (Calact), organ blood flow (radioactive microspheres), and organ vascular resistance in four groups of anesthetized (pentobarbital) male Wistar rats (n = 7 per group), infused from t = 0 to t = 60 min with saline (group C: time matched control), endotoxin Escherichia coli O127:B8, 8 mg.kg-1 (group E), a "low PAF dose" (1 microgram.kg-1) to cause the same decrease in MAP as in group E (group PL), or a "high PAF dose" (3 micrograms.kg-1) to cause the same decrease in CO as in group E (group PH). At t = 60 min, MAP had decreased by 33% in E and PL, and by 55% in PH group. CO had decreased by 41% in the E and PH group. Calact had increased in the E and PH group by 300 and 200%, respectively. In the E, PL and PH group, coronary vascular resistance decreased. In the splanchnic organs, endotoxin caused a decrease in blood flow due to vasoconstriction, whereas PAF (both concentrations) caused vasodilation (except for spleen). Renal vascular resistance decreased (P < 0.05) in the PL group. In all groups, vascular resistance had increased (P < 0.05) in skin, and not changed in skeletal muscle (P < 0.05). Thus, hemodynamic changes after PAF infusion were partially similar to those after endotoxin infusion (coronary vasodilation and vasoconstriction in spleen and skin).(ABSTRACT TRUNCATED AT 250 WORDS)

内毒素或血小板活化因子(PAF)引起的大鼠休克时的全身和局部血流动力学变化。
为了评价血小板活化因子(PAF)在内毒素休克中的作用,我们将其与内毒素的作用进行了比较。我们测量了动脉压(MAP)、心率(HR)、心输出量(CO);四组(戊巴比妥)麻醉雄性Wistar大鼠(每组7只),从t = 0至t = 60 min注射生理盐水(C组:时间匹配对照),内毒素大肠杆菌O127:B8, 8 mg,观察其动脉乳酸(Calact),器官血流(放射性微球)和器官血管阻力。“低PAF剂量”(1微克。公斤。1)导致与E组(PL组)相同的MAP减少,或“高PAF剂量”(3微克。公斤。1)导致与E组(PH组)相同的CO减少。在t = 60 min时,E组和PL组MAP下降33%,PH组下降55%。E和PH组CO降低了41%。E组和PH组的Calact分别增加了300和200%。在E、PL和PH组,冠状动脉血管阻力降低。在内脏器官中,内毒素引起血管收缩导致血流减少,而PAF(两种浓度)引起血管舒张(脾脏除外)。PL组肾血管阻力降低(P < 0.05)。各组皮肤血管阻力升高(P < 0.05),骨骼肌血管阻力无明显变化(P < 0.05)。因此,PAF输注后的血流动力学变化与内毒素输注后的血流动力学变化部分相似(脾脏和皮肤的冠状动脉血管扩张和血管收缩)。(摘要删节250字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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