Voltage clamping of a frog (Rana catesbeiana) taste cell with a single microelectrode.

Abstract

1. Receptor currents in frog taste cells induced by taste stimuli of NaCl and HCl were studied in situ using a voltage clamp method with a single microelectrode. 2. Under voltage clamping of a taste cell at -20 mV an inward receptor current of a mean of 176 pA was induced by taste stimulation with 0.5 M NaCl and the input resistance was largely reduced during the stimulation. 3. A large sustained inward current was evoked by HCl stimulation but the input resistance was slightly reduced. 4. Increasing the holding potential in the negative direction linearly increased the inward receptor current by NaCl stimulation but did not remarkably change it by HCl stimulation.