Immunohistochemical and functional studies on rat high endothelial venules using monoclonal antibodies.

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI:10.1007/BF02899276

M Ohbayashi, M Ito, A Nakayama, K Kasai, J Asai

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引用次数: 2

Abstract

The distribution of high endothelial venules (HEVs) in various rat organs has been investigated immunohistochemically using two monoclonal antibodies (MoAbs), REC16-11 and REC4-1. The staining patterns observed with REC16-11 and REC4-1 were compared with those obtained with the MoAb anti-rat ICAM-1, which is a cell adhesion molecule in HEVs. REC16-11 reacted not only with HEVs but also with all vascular endothelial cells (ECs) in the tissues examined. Electronmicroscopy showed that REC16-11 and REC4-1 reacted with the cell surface antigens of ECs and immunoblot analysis of rat splenic stromal preparations showed that REC4-1 stained 42 kd and 60 kd bands. REC4-1 inhibited the binding of lymphocyte to HEVs in the mucosa-associated lymphoid tissues (MALT), but had no effects on lymphocyte binding to HEVs in peripheral (non-mucosal) lymph nodes. These findings suggested that the MoAb REC4-1 recognized the associated antigen of the lymphocyte-HEV recognition system in MALT.

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单克隆抗体对大鼠高内皮小静脉的免疫组化和功能研究。

利用两种单克隆抗体(MoAbs) REC16-11和REC4-1，免疫组织化学方法研究了高内皮小静脉(HEVs)在大鼠各器官中的分布。REC16-11和REC4-1与MoAb抗大鼠ICAM-1 (hev细胞粘附分子)的染色结果进行了比较。REC16-11不仅能与hev反应，还能与所检查组织中的所有血管内皮细胞(ECs)反应。电镜显示，REC16-11和REC4-1与ECs细胞表面抗原发生反应，大鼠脾基质制剂免疫印迹分析显示，REC4-1染色42 kd和60 kd条带。REC4-1抑制粘膜相关淋巴组织(MALT)淋巴细胞与hev的结合，但对外周(非粘膜)淋巴结淋巴细胞与hev的结合没有影响。这些结果表明，MoAb REC4-1识别MALT中淋巴细胞- hev识别系统的相关抗原。

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Virchows Archiv. B, Cell pathology including molecular pathology

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