Post-translational and transcriptional regulation of polyamine biosynthesis in Escherichia coli

International Journal of Biochemistry Pub Date : 1994-08-01 DOI:10.1016/0020-711X(94)90070-1

Christos A. Panagiotidis, Shu-Ching Huang , Evangelos S. Canellakis

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引用次数: 14

Abstract

1.
1. Ornithine and arginine decarboxylases (ODC and ADC) of Escherichia coli are inhibited post-translationally by antizyme and ribosomal proteins S20 and L34.
2.
2. The inhibition of either enzyme is relieved when excess of the other decarboxylase is added.
3.
3. Using this approach, in vitro as well as in vivo, we demonstrate that the extent of the post-translational inhibition of ODC and ADC in E. coli is at least 65 and 50%, respectively.
4.
4. The inhibited enzyme levels increase even further upon exposure of cells to polyamines.
5.
5. The post-translational mode of regulation can counteract a 4-fold increase of ODC protein in the cell.
6.
6. The negative transcriptional regulation of ODC and ADC expression by polyamines is mediated by transcription factors and not by direct polyamine effects on the promoters of their genes.
7.
7. Three proteins interacting with the ODC promoter region were found by southwestern blot analysis.

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大肠杆菌多胺生物合成的翻译后和转录调控

1.1. 翻译后抗酶和核糖体蛋白S20和L34.2.2抑制了大肠杆菌鸟氨酸和精氨酸脱羧酶(ODC和ADC)的活性。当加入过量的另一种脱羧酶时，任一种酶的抑制作用都会减轻。使用这种方法，在体外和体内，我们证明了ODC和ADC在大肠杆菌中的翻译后抑制程度分别至少为65%和50%。当细胞暴露于多胺时，抑制酶的水平甚至进一步增加。翻译后的调节模式可以抵消细胞中ODC蛋白4倍的增加。多胺对ODC和ADC表达的负转录调控是由转录因子介导的，而不是多胺对其基因启动子的直接作用。通过西南印迹分析发现了3个与ODC启动子区相互作用的蛋白。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Biochemistry

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