Substrate utilization by Rana ridibunda erythrocytes

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-07-01 DOI:10.1016/0305-0491(94)90087-6

Martha Kaloyianni, Katerina Moutou

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引用次数: 3

Abstract

Various monosaccharides, including ribose, mannose, galactose, and urea, in combination with glucose, were studied to determine their efficacy in supporting the formation of pyruvate, lactate, 2,3-diphosphoglycerate and ATP in Rana ridibunda erythrocytes. Lactate formation was found to increase during the course of incubation in the presence of all the substrates. None of the studied substrates maintained cellular ATP levels. About 0.36 μmole of lactic acid per hour was produced for each μmole of ribose that was metabolized. The presence of 1 mM Na-iodoacetate accelerated the loss of ATP and lactate in the presence of either glucose or ribose. Additionally, ouabain suppressed lactate formation from ribose alone, as well as in combination with glucose. From the metabolic substrates studied, ribose was shown to be the most efficient substrate to support Rana ridibunda erythrocyte metabolism. Mannose, galactose and urea may also be used as alternative metabolic substrates by Rana ridibunda erythrocytes.

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蛙红细胞对底物的利用

研究了各种单糖，包括核糖、甘露糖、半乳糖和尿素，与葡萄糖结合，以确定它们在支持核糖核糖红细胞中丙酮酸、乳酸、2,3-二磷酸甘油酸和ATP形成的功效。发现在所有底物存在的孵育过程中乳酸形成增加。所研究的底物均未维持细胞ATP水平。每代谢1 μmol核糖，每小时产生约0.36 μmol乳酸。在葡萄糖或核糖存在的情况下，1mm na -碘乙酸加速ATP和乳酸的损失。此外，瓦巴因单独抑制核糖以及与葡萄糖联合抑制乳酸形成。从所研究的代谢底物中，核糖被证明是支持蛙红细胞代谢最有效的底物。甘露糖、半乳糖和尿素也可作为瑞金蛙红细胞的替代代谢底物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

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